10-hydroxy-cis-12 octadecenoic acid (10-HOE) is a type of octadecenoic acid with a hydroxyl on the C10 carbon. It is generated from linoleic acid (LA) catalyzed by linoleate hydratase in lactobacilli, which was initially named as myosin-cross-reactive antigen (MCRA). In lactobacilli, 10-HOE is the first intermediate in the production of conjugated LA (CLA). Although MCRA from bifidobacteria can generate 10-HOE, the precise role of 10-HOE in CLA production in bifidobacteria remains unknown. In the current work, 10-HOE and LA were added to the medium as the substrate both separately and synchronously to analyze their influence on CLA production. Using 10-HOE as the substrate, bifidobacteria were able to generate CLA by first converting it to LA, followed by CLA accumulation. Recombinant MCRA catalyzed the conversion of 10-HOE to LA, indicating that bifidobacterial MCRA can account for the reversible conversion between LA and 10-HOE. This is the first report to demonstrate the precise role of 10-HOE in the process of CLA production among bifidobacteria.

中文翻译：

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10-羟基-顺式-12-十八碳烯酸在双歧杆菌将亚油酸转化为共轭亚油酸中的作用。

10-羟基-顺式-12十八碳烯酸（10-HOE）是一种十八碳烯酸，在C10碳上带有羟基。它由乳酸杆菌中的亚油酸水合酶催化的亚油酸（LA）生成，最初被称为肌球蛋白交叉反应抗原（MCRA）。在乳酸杆菌中，10-HOE是共轭LA（CLA）生产中的第一个中间体。尽管双歧杆菌的MCRA可以产生10-HOE，但10-HOE在双歧杆菌的CLA生产中的确切作用仍然未知。在当前的工作中，分别和同步地将10-HOE和LA作为底物添加到介质中，以分析它们对CLA生产的影响。使用10-HOE作为底物，双歧杆菌能够通过首先将其转化为LA，然后进行CLA积累来生成CLA。重组MCRA催化了10-HOE向LA的转化，表明双歧杆菌MCRA可以解释LA和10-HOE之间的可逆转化。这是第一份证明10-HOE在双歧杆菌CLA生产过程中确切作用的报告。