1-Aminocyclopropane-1-Carboxylate：一种新型和强大的趋化性植物有益假单胞菌恶臭假单胞菌UW4。,Molecular Plant-Microbe Interactions

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1-Aminocyclopropane-1-Carboxylate：一种新型和强大的趋化性植物有益假单胞菌恶臭假单胞菌UW4。
Molecular Plant-Microbe Interactions ( IF 3.2 ) Pub Date : 2019-01-15 , DOI: 10.1094/mpmi-11-18-0317-r
Tao Li ₁ , Jun Zhang ₁ , Chaohui Shen ₁ , Huiru Li ₁ , Liyou Qiu ₁

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促进植物生长的根际细菌（PGPR）和真菌细菌生物膜都是可持续农业的重要生物肥料孕育剂。然而，尚不清楚细菌在根际和菌丝体中定植的最强化学吸引剂。巧合的是，几乎所有的PGPR都具有1-氨基环丙烷-1-羧酸酯（ACC）脱氨酶（AcdS），并且可以利用ACC作为唯一的氮源。在这里，我们发现ACC是恶臭假单胞菌（Pseudomonas putida）UW4的一种新的，代谢依赖性且受甲基接受的化学感受器。UW4对ACC的化学趋化响应明显大于对植物根和真菌菌丝渗出物中鉴定出的氨基酸和有机酸的趋化响应。与UW4相比，在小麦根际和双孢蘑菇菌丝体上的UW4 acdS或cheR缺失突变体的定殖数减少了一个数量级。与双孢曲霉相比，双孢曲霉反义ACC氧化酶菌丝体上UW4的定殖计数与双孢曲霉相比明显增加，其次是UW4 cheR互补菌株和乙烯化学感受器基因缺失突变体。与野生双孢曲霉相比，UW4菌株在双孢曲霉acdS +菌丝体上的ACC产量较低的定殖计数显着降低。这些结果表明，对于含有AcdS的PGPR，ACC而非乙烯应该是最强的化学吸引剂。与双孢曲霉相比，具有高ACC产量的双孔反义ACC氧化酶菌丝体显着增加，其次是UW4 cheR互补菌株和乙烯化学感受器基因缺失突变体。与野生双孢曲霉相比，UW4菌株在双孢曲霉acdS +菌丝体上的ACC产量较低的定殖计数显着降低。这些结果表明，对于含有AcdS的PGPR，ACC而非乙烯应该是最强的化学吸引剂。与双孢曲霉相比，具有高ACC产量的双孔反义ACC氧化酶菌丝体显着增加，其次是UW4 cheR互补菌株和乙烯化学感受器基因缺失突变体。与野生双孢曲霉相比，UW4菌株在双孢曲霉acdS +菌丝体上的AW产量较低的定殖计数显着降低。这些结果表明，对于含有AcdS的PGPR，ACC而非乙烯应该是最强的化学吸引剂。

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1-Aminocyclopropane-1-Carboxylate: A Novel and Strong Chemoattractant for the Plant Beneficial Rhizobacterium Pseudomonas putida UW4.

Plant growth-promoting rhizobacteria (PGPR) and fungi-bacterial biofilms are both important biofertilizer inoculants for sustainable agriculture. However, the strongest chemoattractant for bacteria to colonize the rhizosphere and mycelia is not clear. Coincidentally, almost all the PGPRs possess 1-aminocyclopropane-1-carboxylate (ACC) deaminase (AcdS) and can utilize ACC as the sole nitrogen source. Here, we found that ACC was a novel, metabolic dependent and methyl-accepting chemoreceptor-involved chemoattractant for Pseudomonas putida UW4. The chemotactic response of UW4 to ACC is significantly greater than that to the amino acids and organic acids identified in the plant root and fungal hyphal exudates. The colonization counts of the UW4 acdS or cheR deletion mutants in the wheat rhizosphere and on Agaricus bisporus mycelia were reduced one magnitude compared with those of UW4. The colonization counts of UW4 on A. bisporus antisense ACC oxidase mycelia with a high ACC production significantly increased compared with A. bisporus, followed by the UW4 cheR complementary strain and the ethylene chemoreceptor gene-deletion mutant. The colonization counts of the UW4 strains on A. bisporus acdS+ mycelia with a low ACC production decreased significantly compared with A. bisporus wild type. These results suggested that ACC and not ethylene should be the strongest chemoattractant for the PGPR that contain AcdS.

更新日期：2019-11-01

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