GS-CHO细胞对单磷酸腺苷处理的生理变化。,Journal of Biotechnology

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GS-CHO细胞对单磷酸腺苷处理的生理变化。
Journal of Biotechnology ( IF 4.1 ) Pub Date : 2019-02-16 , DOI: 10.1016/j.jbiotec.2019.01.016
Wu Zou ₁ , Susan M Browne ₁ , Mohamed Al-Rubeai ₂

Affiliation

在哺乳动物细胞培养生物过程中，生长停滞策略（例如，体温过低和高渗）已被广泛用于增强细胞比生产率（qP）。除了增强的qP以外，在生长停滞的条件下，细胞生理学的改变（例如细胞大小和细胞周期阶段）也引起了广泛的关注。然而，迄今为止，关于生长抑制方法中的生理变化之间的关联的报道很少。在这项研究中，我们探讨了响应单磷酸腺苷（AMP）处理的GS-CHO细胞生理变化之间的关联。在剂量反应研究中，AMP治疗导致增殖抑制，S期细胞积累，细胞大小增加和qP增强。后来，当用7 mM AMP处理时，六个GS-CHO克隆表现出不同程度的生理变化。但更重要的是，发现总细胞内蛋白质含量与平均电子体积之间呈显着正相关，这是细胞大小的指标（P <0.01），表明在这种生长停滞策略中，总细胞内蛋白质是增加细胞大小的决定因素。此外，我们的结果提供了进一步的证据，证明用生长停滞剂治疗可能会增加细胞大小。该试剂本身并没有导致生产率的提高。表明在这种生长抑制策略中，总细胞内蛋白是增加细胞大小的决定因素。此外，我们的结果提供了进一步的证据，证明用生长停滞剂治疗可能会增加细胞大小。该试剂本身并没有导致生产率的提高。表明在这种生长抑制策略中，总细胞内蛋白是增加细胞大小的决定因素。此外，我们的结果提供了进一步的证据，证明用生长停滞剂治疗可能会增加细胞大小。该试剂本身并没有导致生产率的提高。

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Physiological alterations of GS-CHO cells in response to adenosine monophosphate treatment.

Growth-arrested strategies (e.g. hypothermia and hyperosmolarity) have been widely employed to enhance cell-specific productivity (qP) in mammalian cell culture bioprocess. In addition to enhanced qP, alterations in cell physiology, such as cell size and cell cycle phase, have also attracted extensive attention under growth-arrested conditions. However, to date, very few reports on associations between physiological changes in growth-inhibiting approaches have been published. In this study, we explored associations between the physiological changes of GS-CHO cells in response to adenosine monophosphate (AMP) treatment. In dose response studies, AMP treatment resulted in suppressed proliferation, accumulated S-phase cells, increased cell size and enhanced qP. Subsequently, six GS-CHO clones exhibited the physiological alterations in varying degrees when treated with 7 mM AMP. But more importantly, a significant positive correlation between total intracellular protein content and mean electronic volume, an indicator of cell size (P < 0.01) was found, indicating that total intracellular protein was the determining factor in increasing cell size in this growth-arrested strategy. Besides, our results provide additional evidence that treatment with growth-arrested agents may increase cell size; the agent per se did not cause the increased productivity.

更新日期：2019-11-01

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