In this work, the chemiluminescence method was engaged for the first time to detect the active oxygen species during sonodynamic action in vitro and in vivo. We used FCLA (3,7-dihydro-6-[4-[2-[N'-(5-fluoresceinyl)thioureido]-ethoxy]phenyl]-2-methylimidazo[1,2-a]pyrazin-3-one sodium salt), which can selectively react with singlet oxygen ((1)O(2)) or superoxide anion (O(2)(-)) to emit photons, to detect in real time oxygen free radical formation in the sonosensitization of hematoporphyrin derivative. The results show that (1)O(2) is involved in the sonosensitization. In in vivo experiments, a tumor-imaging method by sonodynamic chemiluminescence detection was established. This method could have potential applications in clinics for tumor diagnosis.

中文翻译：

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体外和体内声动力学作用产生的FCLA化学发光。

在这项工作中，首次采用化学发光方法在体外和体内声动力学作用期间检测活性氧。我们使用了FCLA（3,7-二氢-6- [4- [2- [N'-（5-荧光基）硫脲基]-乙氧基]苯基] -2-甲基咪唑并[1,2-a]吡嗪-3-一钠盐），可以选择性地与单线态氧（（1）O（2））或超氧阴离子（O（2）（-））反应以发射光子，以实时检测血卟啉的声敏性中的氧自由基形成衍生物。结果表明（1）O（2）参与声致敏化。在体内实验中，建立了通过声动力学化学发光检测的肿瘤成像方法。该方法在临床诊断肿瘤中可能具有潜在的应用价值。