编码2-酮-3-脱氧葡萄糖酸通透酶的欧文氏菊基因的核苷酸序列。,Gene

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编码2-酮-3-脱氧葡萄糖酸通透酶的欧文氏菊基因的核苷酸序列。
Gene ( IF 2.6 ) Pub Date : 1989-11-30 , DOI: 10.1016/0378-1119(89)90109-1
C Allen ₁ , S Reverchon , J Robert-Baudouy

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植物病原菌菊花欧文氏菌（Erwinia chrysanthemi）产生一组果胶分解酶，能够解聚植物细胞壁中的果胶化合物。导致的组织浸软被称为软腐病。降解的果胶产物通过2-酮-3-脱氧葡萄糖酸酯渗透酶转运到细菌细胞中，在细菌细胞中用作碳和能源。这种H +偶联的转运系统由kdgT基因编码；我们报告了kdgT的核苷酸序列。它由一个1194 bp的开放阅读框（ORF）编码，之后是一个大肠杆菌型启动子区域。ORF编码具有398个氨基酸（aa）残基且预测的Mr为48,550的蛋白质。正如对膜蛋白所期望的那样，它非常疏水，含有63％的非极性氨基酸。然而，kdgT基因与其他编码糖转运蛋白的基因（如lacY，melB或大肠杆菌柠檬酸转运基因）没有明显的进化关系。Southern杂交实验表明Er之间有很强的同源性。菊花和大肠杆菌的kdgT基因；在大肠杆菌染色体上还有与kdgT同源的第二个区域。kdgT基因位于Er的ade-377标记附近。chrysanthemi染色体（相当于大肠杆菌中20至30分钟之间的区域），而大肠杆菌kdgT基因位于88分钟处。因此，这两种肠杆菌在其基因组组织上显示出一些显着差异。在大肠杆菌染色体上还有与kdgT同源的第二个区域。kdgT基因位于Er的ade-377标记附近。chrysanthemi染色体（相当于大肠杆菌中20至30分钟之间的区域），而大肠杆菌kdgT基因位于88分钟处。因此，这两种肠杆菌在其基因组组织上显示出一些显着差异。在大肠杆菌染色体上还有与kdgT同源的第二个区域。kdgT基因位于Er的ade-377标记附近。chrysanthemi染色体（相当于大肠杆菌中20至30分钟之间的区域），而大肠杆菌kdgT基因位于88分钟处。因此，这两种肠杆菌在其基因组组织上显示出一些显着差异。

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Nucleotide sequence of the Erwinia chrysanthemi gene encoding 2-keto-3-deoxygluconate permease.

The phytopathogenic bacterium Erwinia chrysanthemi produces a group of pectolytic enzymes able to depolymerise the pectic compounds in plant cell walls. The resulting tissue maceration is known as soft rot disease. The degraded pectin products are transported by 2-keto-3-deoxygluconate permease into the bacterial cell, where they serve as carbon and energy sources. This H+ coupled transport system is encoded by the kdgT gene; we report the nucleotide sequence of kdgT. It is encoded by an open reading frame (ORF) of 1194 bp, which is preceded by an Escherichia coli-type promoter region. The ORF encodes a protein with 398 amino acid (aa) residues and a predicted Mr of 48,550. As would be expected for a membrane protein, it is very hydrophobic, containing 63% nonpolar aa. However, the kdgT gene has no apparent evolutionary relationship to other genes encoding sugar transport proteins, such as lacY, melB or the E. coli citrate transport gene. Southern hybridization experiments indicate a strong homology between the Er. chrysanthemi and E. coli kdgT genes; there is also a second region on the E. coli chromosome with homology to kdgT. The kdgT gene is located near the ade-377 marker on the Er. chrysanthemi chromosome (equivalent to the region between 20 and 30 min in E. coli), whereas the E. coli kdgT gene is located at 88 min. Thus, these two enterobacteria show some significant differences in their genomic organization.

更新日期：2019-11-01

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