生物碱taspine定义作用机制，促进伤口愈合。,Experimental Biology and Medicine

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生物碱taspine定义作用机制，促进伤口愈合。
Experimental Biology and Medicine ( IF 2.8 ) Pub Date : 1993-05-01 , DOI: 10.3181/00379727-203-43567
B H Porras-Reyes ₁ , W H Lewis , J Roman , L Simchowitz , T A Mustoe

Affiliation

Taspine（mol wt 369,000）是从亚马逊河西部地区的巴豆（大戟科）的树木中提取的一种生物碱，已被当地人和其他人用作致病菌。从树液中提纯Taspine，以在成对的大鼠手术切口模型中使用不同的局部浓度测试其愈合性能。评估伤口的抗张强度和组织学。用250微克处理的样品，而不是用50微克或10微克处理的样品，其MBS值显着高于配对对照（分别在第5天和第7天分别为26％，P <0.005和30％，P <0.001）。。Taspine在第12天没有改变MBS。用250微克处理的样品在第5天和第7天具有明显更大的单核细胞浸润，但在第12天没有。为了更好地了解Taspine作为伤口愈合促进剂的作用，我们在细胞培养物中进行了体外研究。Taspine刺激成纤维细胞趋化性。Taspine对巨噬细胞趋化性，嗜中性粒细胞活化，成纤维细胞增殖或基质组装的特定测定没有影响。两者合计，数据表明taspine以剂量依赖性方式促进伤口愈合的早期阶段，此后没有实质性改变。它的作用机制可能与其对成纤维细胞的趋化特性有关，而不是由细胞外基质的变化介导的。数据表明，taspine以剂量依赖性方式促进伤口愈合的早期阶段，此后没有任何实质性改变。它的作用机制可能与其对成纤维细胞的趋化特性有关，而不是由细胞外基质的变化介导的。数据表明，taspine以剂量依赖性方式促进伤口愈合的早期阶段，此后没有任何实质性改变。它的作用机制可能与其对成纤维细胞的趋化特性有关，而不是由细胞外基质的变化介导的。

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Enhancement of wound healing by the alkaloid taspine defining mechanism of action.

Taspine (mol wt 369,000) is an alkaloid extracted from trees of Croton (family Euphorbiaceae) of the western Amazon region that has been used by natives and others as a vulnerary agent. Taspine was purified from tree sap to test its healing properties using different topical concentrations in the paired rat surgical incision model. Wound tensile strength and histology were evaluated. Samples treated with 250 micrograms, but not those treated with 50 micrograms or 10 micrograms, had significant higher values for MBS than paired controls (26%, P < 0.005, and 30%, P < 0.001, by Days 5 and 7, respectively). Taspine did not modify MBS at Day 12. Sample treated with 250 micrograms had significantly greater mononuclear cellular infiltration at Days 5 and 7 but not at Day 12. To better understand the effect of taspine as an enhancer of wound healing, we conducted in vitro studies in cell cultures. Taspine stimulated chemotaxis for fibroblasts. Taspine did not have an effect on specific assays for macrophage chemotaxis, neutrophil activation, fibroblast proliferation, or matrix assembly. Taken together, the data suggest that taspine promotes early phases of wound healing in a dose-dependent manner with no substantial modification thereafter. Its mechanism of action is probably related to its chemotactic properties on fibroblasts and is not mediated by changes in extracellular matrix.

更新日期：2019-11-01

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