Abstract4-Methyl-5-pentylbenzene-1,3-diol (MPBD), a product of the polyketide synthase SteelyA, is a signaling molecule that regulates Dictyostelium discoideum development. During early development, MPBD controls chemotactic cell aggregation by regulating the expression of genes in the cAMP signaling pathway; however, during culmination at late development, it induces spore maturation. In the present study, we analyzed the effects of MPBD, its derivatives, and a putative MPBD-derived metabolite on developmental defects in the MPBD-less stlA null mutant. Using structure–activity relationship studies, it was observed that in MPBD, the functional groups that were essential for induction of spore maturation were different from those essential for induction of cell aggregation. Dictyoquinone, a putative MPBD metabolite rescued the aggregation defect in stlA null mutant in early development, but not the spore maturation defect at the later stage. Our data suggest that MPBD regulates chemotactic cell aggregation and spore maturation via different mechanisms.

中文翻译：

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4-Methyl-5-Pentylbenzo-1,3-Diol 通过不同机制调节盘基网柄菌的趋化细胞聚集和孢子成熟

摘要 4-甲基-5-戊基苯-1,3-二醇 (MPBD) 是聚酮化合物合成酶 SteelyA 的产物，是调节盘基网柄菌发育的信号分子。在早期发育过程中，MPBD 通过调节 cAMP 信号通路中基因的表达来控制趋化细胞聚集；然而，在发育后期达到顶峰时，它会诱导孢子成熟。在本研究中，我们分析了 MPBD、其衍生物和推定的 MPBD 衍生代谢物对 MPBD-less stlA 空突变体发育缺陷的影响。使用构效关系研究，观察到在 MPBD 中，诱导孢子成熟所必需的官能团与诱导细胞聚集所必需的官能团不同。丁二酮，假定的 MPBD 代谢物在早期发育中挽救了 stlA 无效突变体中的聚集缺陷，但在后期没有挽救孢子成熟缺陷。我们的数据表明 MPBD 通过不同的机制调节趋化细胞聚集和孢子成熟。