CD66a，CD66b，CD66c和CD66d各自独立刺激中性粒细胞。,Journal of Leukocyte Biology

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CD66a，CD66b，CD66c和CD66d各自独立刺激中性粒细胞。
Journal of Leukocyte Biology ( IF 3.6 ) Pub Date : 1996-07-01 , DOI: 10.1002/jlb.60.1.106
K M Skubitz ₁ , K D Campbell , A P Skubitz

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癌胚抗原家族的四个成员CD66a，CD66b，CD66c和CD66d在人嗜中性粒细胞上表达。在嗜中性粒细胞中，这些蛋白质是激活抗原，因为它们的表面表达在刺激后会增加。为了检查其在嗜中性粒细胞信号传导中的潜在作用，测试了一组特征明确的CD66 mAb对嗜中性粒细胞粘附于人脐静脉内皮细胞的影响。CD66a，CD66b，CD66c和CD66d抗体各自增加嗜中性粒细胞对人脐静脉内皮细胞单层的粘附。由CD66抗体引起的嗜中性粒细胞粘附的这种增加被CD18抗体阻断，并与嗜中性粒细胞表面CD11 / CD18的上调相关。中性粒细胞粘附的这种增加需要在CD66抗体与中性粒细胞结合时或接近该时间时的生理细胞外钙浓度。在没有钙的情况下，将CD66抗体与中性粒细胞孵育10分钟，然后再补充钙，从而不会增加中性粒细胞的粘附。数据表明与中性粒细胞表面结合的CD66a，CD66b，CD66c和CD66d抗体会触发一个瞬态激活信号，该信号需要细胞外钙并调节CD11 / CD18的黏附活性。顺序脱敏实验表明，CD66a，CD66b，CD66c和CD66d可以各自独立地在嗜中性粒细胞中传递信号。在没有钙的情况下，将CD66抗体与中性粒细胞孵育10分钟，然后再补充钙，从而不会增加中性粒细胞的黏附力。数据表明与中性粒细胞表面结合的CD66a，CD66b，CD66c和CD66d抗体会触发一个瞬态激活信号，该信号需要细胞外钙并调节CD11 / CD18的黏附活性。顺序脱敏实验表明，CD66a，CD66b，CD66c和CD66d可以各自独立地在嗜中性粒细胞中传递信号。在没有钙的情况下，将CD66抗体与中性粒细胞孵育10分钟，然后再补充钙，从而不会增加中性粒细胞的粘附。数据表明与中性粒细胞表面结合的CD66a，CD66b，CD66c和CD66d抗体会触发一个瞬态激活信号，该信号需要细胞外钙并调节CD11 / CD18的粘附活性。顺序脱敏实验表明，CD66a，CD66b，CD66c和CD66d可以各自独立地在嗜中性粒细胞中传递信号。

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CD66a, CD66b, CD66c, and CD66d each independently stimulate neutrophils.

Four members of the carcinoembryonic antigen family, CD66a, CD66b, CD66c, and CD66d, are expressed on human neutrophils. In neutrophils these proteins are activation antigens in that their surface expression is increased following stimulation. To examine their potential role in neutrophil signaling, the effects on neutrophil adhesion to human umbilical vein endothelial cells of a panel of well-characterized CD66 mAbs was tested. CD66a, CD66b, CD66c, and CD66d antibodies each increased neutrophil adhesion to human umbilical vein endothelial cell monolayers. This increase in neutrophil adhesion caused by CD66 antibodies was blocked by a CD18 antibody and associated with up-regulation of CD11/CD18 on the neutrophil surface. This increase in neutrophil adhesion required physiological extracellular calcium concentrations at or near the time of CD66 antibody binding to the neutrophil. The incubation of CD66 antibodies with neutrophils in the absence of calcium for 10 min before repletion of calcium resulted in no increase in neutrophil adhesion. The data suggest that CD66a, CD66b, CD66c, and CD66d antibody binding to the neutrophil surface triggers a transient activation signal that requires extracellular calcium and regulates the adhesive activity of CD11/CD18. Sequential desensitization experiments indicated that CD66a, CD66b, CD66c, and CD66d can each independently transmit signals in neutrophils.

更新日期：2019-11-01

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