大肠杆菌MG1655菌株的生理研究：生长缺陷和基因表达的明显交叉调节。,Journal of Bacteriology

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大肠杆菌MG1655菌株的生理研究：生长缺陷和基因表达的明显交叉调节。
Journal of Bacteriology ( IF 2.7 ) Pub Date : 2003-09-02 , DOI: 10.1128/jb.185.18.5611-5626.2003
Eric Soupene ₁ , Wally C van Heeswijk , Jacqueline Plumbridge , Valley Stewart , Daniel Bertenthal , Haidy Lee , Gyaneshwar Prasad , Oleg Paliy , Parinya Charernnoppakul , Sydney Kustu

Affiliation

选择大肠杆菌菌株MG1655进行测序，因为它携带的少数突变（ilvG rfb-50 rph-1）被认为是无害的。但是，它具有许多生长缺陷。由于pyrE上的rph-1等位基因的极性，内部嘧啶饥饿在连续培养中存在问题。此外，从大肠杆菌遗传储备中心获得的MG1655分离株在fnr（富马酸-硝酸盐呼吸）调节基因周围也带有大量缺失。尽管对DNA微阵列的研究表明MG1655的Stock Center分离株中半乳糖和乳糖代谢之间的基因表达存在明显的交叉调节，但这是由于发生了增加lacY表达并抑制半乳糖缓慢生长的突变。半乳糖和N-乙酰氨基葡萄糖代谢之间明显的交叉调节的解释是相似的。相比之下，乳糖和麦芽糖代谢之间的交叉调节似乎是由于乳糖生长的细胞中内部麦芽糖的产生，在生理上可能很重要。乳糖的分布受到限制：通常在哺乳动物的肠道中发现它与淀粉降解产物麦芽糖一道被发现。从其他来源获得的命名为MG1655的菌株与斯托克中心分离株在几个方面有所不同。我们证实，将其他大肠杆菌菌株与基于MG1655的DNA微阵列配合使用效果很好，因此这些阵列可用于研究任何目标菌株。

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Physiological studies of Escherichia coli strain MG1655: growth defects and apparent cross-regulation of gene expression.

Escherichia coli strain MG1655 was chosen for sequencing because the few mutations it carries (ilvG rfb-50 rph-1) were considered innocuous. However, it has a number of growth defects. Internal pyrimidine starvation due to polarity of the rph-1 allele on pyrE was problematic in continuous culture. Moreover, the isolate of MG1655 obtained from the E. coli Genetic Stock Center also carries a large deletion around the fnr (fumarate-nitrate respiration) regulatory gene. Although studies on DNA microarrays revealed apparent cross-regulation of gene expression between galactose and lactose metabolism in the Stock Center isolate of MG1655, this was due to the occurrence of mutations that increased lacY expression and suppressed slow growth on galactose. The explanation for apparent cross-regulation between galactose and N-acetylglucosamine metabolism was similar. By contrast, cross-regulation between lactose and maltose metabolism appeared to be due to generation of internal maltosaccharides in lactose-grown cells and may be physiologically significant. Lactose is of restricted distribution: it is normally found together with maltosaccharides, which are starch degradation products, in the mammalian intestine. Strains designated MG1655 and obtained from other sources differed from the Stock Center isolate and each other in several respects. We confirmed that use of other E. coli strains with MG1655-based DNA microarrays works well, and hence these arrays can be used to study any strain of interest. The responses to nitrogen limitation of two urinary tract isolates and an intestinal commensal strain isolated recently from humans were remarkably similar to those of MG1655.

更新日期：2019-11-01

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