p22phox是产生超氧化物的NADH / NADPH氧化酶系统的关键组成部分，可调节血管紧张素II诱导的血管平滑肌细胞肥大。,Journal of Biological Chemistry

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p22phox是产生超氧化物的NADH / NADPH氧化酶系统的关键组成部分，可调节血管紧张素II诱导的血管平滑肌细胞肥大。
Journal of Biological Chemistry ( IF 4.0 ) Pub Date : 1996-09-20 , DOI: 10.1074/jbc.271.38.23317
M Ushio-Fukai ₁ , A M Zafari , T Fukui , N Ishizaka , K K Griendling

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超氧阴离子的形成对于吞噬细胞的杀微生物活性至关重要。但是，最近有越来越多的证据表明它也参与血管平滑肌细胞（VSMC）的细胞生长。我们已经表明，肥大剂血管紧张素II通过激活膜结合的NADH / NADPH氧化酶来刺激超氧化物的产生，并且对该氧化酶的抑制会减弱血管肥大。但是，尚不清楚该氧化酶在VSMC中的分子身份。我们最近从大鼠VSMC cDNA库中克隆了细胞色素b558α亚基p22（phox）（吞噬细胞中NADPH氧化酶的关键电子转移元素之一），但尚不清楚它在VSMC氧化酶活性中的作用。在这里我们报道了通过将反义p22（phox）cDNA稳定转染到VSMCs中来完全抑制p22（phox）mRNA的表达会导致细胞色素b含量的减少，同时伴随着血管紧张素II刺激的NADH / NADPH的显着抑制依赖的超氧化物的产生，随后的过氧化氢的产生以及[3H]亮氨酸的掺入。我们提供的第一个证据表明，p22（phox）是产生超氧化物的血管NADH / NADPH氧化酶的关键成分，并暗示该氧化酶系统在血管肥大中的重要作用。

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p22phox is a critical component of the superoxide-generating NADH/NADPH oxidase system and regulates angiotensin II-induced hypertrophy in vascular smooth muscle cells.

Superoxide anion formation is vital to the microbicidal activity of phagocytes. Recently, however, there is accumulating evidence that it is also involved in cell growth in vascular smooth muscle cells (VSMCs). We have shown that the hypertrophic agent angiotensin II stimulates superoxide production by activating the membrane-bound NADH/NADPH oxidase and that inhibition of this oxidase attenuates vascular hypertrophy. However, the molecular identity of this oxidase in VSMCs is unknown. We have recently cloned the cytochrome b558 alpha-subunit, p22(phox) (one of the key electron transfer elements of the NADPH oxidase in phagocytes), from a rat VSMC cDNA library, but its role in VSMC oxidase activity remains unclarified. Here we report that the complete inhibition of p22(phox) mRNA expression by stable transfection of antisense p22(phox) cDNA into VSMCs results in a decrease in cytochrome b content, which is accompanied by a significant inhibition of angiotensin II-stimulated NADH/NADPH-dependent superoxide production, subsequent hydrogen peroxide production, and [3H]leucine incorporation. We provide the first evidence that p22(phox) is a critical component of superoxide-generating vascular NADH/NADPH oxidase and suggest a central role for this oxidase system in vascular hypertrophy.

更新日期：2019-11-01

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