AdipoQ是肥胖中失调的新型脂肪特异性基因。,Journal of Biological Chemistry

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AdipoQ是肥胖中失调的新型脂肪特异性基因。
Journal of Biological Chemistry ( IF 4.0 ) Pub Date : 1996-05-03 , DOI: 10.1074/jbc.271.18.10697
E Hu ₁ , P Liang , B M Spiegelman

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脂肪分化伴随着细胞形态的变化，细胞内脂质的大量积累和特定基因表达程序的激活。使用mRNA差异显示技术，我们已经分离出一种称为adipoQ的新型脂肪cDNA。adipoQ cDNA编码一个247个氨基酸的多肽，在氨基末端具有一个分泌信号序列，一个胶原蛋白区域（Gly-XY重复序列）和一个球形结构域。adipoQ的球状结构域与补体因子C1q，胶原蛋白alpha 1（X）和脑特异性因子小脑素的亚基具有显着同源性。adipoQ的表达对小鼠和大鼠的脂肪组织都高度特异性。仅在成熟的脂肪细胞中观察到adipoQ的表达，因为脂肪组织的基质血管部分不包含adipoQ mRNA。在培养的3T3-F442A和3T3-L1前脂肪细胞中，激素诱导的分化显着提高了adipoQ的表达水平。此外，在肥胖小鼠和人类的脂肪组织中，adipoQ mRNA的表达显着降低。尽管目前尚不清楚该多肽的生物学功能，但推测的分泌蛋白的组织特异性表达表明该因子可能充当脂肪组织的新型信号分子。

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AdipoQ is a novel adipose-specific gene dysregulated in obesity.

Adipose differentiation is accompanied by changes in cellular morphology, a dramatic accumulation of intracellular lipid and activation of a specific program of gene expression. Using an mRNA differential display technique, we have isolated a novel adipose cDNA, termed adipoQ. The adipoQ cDNA encodes a polypeptide of 247 amino acids with a secretory signal sequence at the amino terminus, a collagenous region (Gly-X-Y repeats), and a globular domain. The globular domain of adipoQ shares significant homology with subunits of complement factor C1q, collagen alpha 1(X), and the brain-specific factor cerebellin. The expression of adipoQ is highly specific to adipose tissue in both mouse and rat. Expression of adipoQ is observed exclusively in mature fat cells as the stromal-vascular fraction of fat tissue does not contain adipoQ mRNA. In cultured 3T3-F442A and 3T3-L1 preadipocytes, hormone-induced differentiation dramatically increases the level of expression for adipoQ. Furthermore, the expression of adipoQ mRNA is significantly reduced in the adipose tissues from obese mice and humans. Whereas the biological function of this polypeptide is presently unknown, the tissue-specific expression of a putative secreted protein suggests that this factor may function as a novel signaling molecule for adipose tissue.

更新日期：2019-11-01

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