GNS1的克隆和表征：酿酒酵母基因，参与体外1,3-β-葡聚糖的合成。,Journal of Bacteriology

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GNS1的克隆和表征：酿酒酵母基因，参与体外1,3-β-葡聚糖的合成。
Journal of Bacteriology ( IF 2.7 ) Pub Date : 1995-06-01 , DOI: 10.1128/jb.177.11.3227-3234.1995
M el-Sherbeini ₁ , J A Clemas

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GNS1基因产物是体外合成1,3-β-葡聚糖所需的，因为该基因的突变导致1,3-β-葡聚糖合酶比活性降低80％至90％。gns1突变菌株表现出多效性表型，包括对肺炎链菌素B0类似物（L-733,560）的抗性，生长缓慢以及交配和孢子形成缺陷。gns1-1突变被遗传定位到染色体III上MAT基因座的1.35厘摩范围内。通过互补gns1-1突变的肺炎链菌素抗性表型，并与来源于染色体III的序列杂交作为探针，分离出野生型GNS1基因。确定了GNS1的核苷酸序列，并将其与染色体的同源区域进行比较。遗传和核苷酸序列分析显示，GNS1和开放阅读框YCR34 [S. 奥利弗（Oliver，Q. van der Aart），阿戈斯托尼（M. Agostoni-Carbone）和染色体III测序小组，自然（伦敦）357：38-46，1992]代表基因组中相同的基因座。为GNS1删除的细胞是可行的，但表现出缓慢的生长以及gns1突变体的多效性表型。推定的蛋白质产物预计将是一个完整的膜蛋白，具有五个跨膜螺旋，对N末端显示胞质外向，对C末端显示胞质外向。该蛋白可以是1，3-β-葡聚糖合酶的亚基。为GNS1删除的细胞是可行的，但表现出缓慢的生长以及gns1突变体的多效性表型。推定的蛋白质产物预计将是一个完整的膜蛋白，具有五个跨膜螺旋，对N末端显示胞质外向，对C末端显示胞质外向。该蛋白可以是1，3-β-葡聚糖合酶的亚基。为GNS1删除的细胞是可行的，但表现出缓慢的生长以及gns1突变体的多效性表型。推定的蛋白质产物预计将是一个完整的膜蛋白，具有五个跨膜螺旋，对N末端显示胞质外向，对C末端显示胞质外向。该蛋白可以是1，3-β-葡聚糖合酶的亚基。

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Cloning and characterization of GNS1: a Saccharomyces cerevisiae gene involved in synthesis of 1,3-beta-glucan in vitro.

The GNS1 gene product is required for the synthesis of 1,3-beta-glucan in vitro, since mutations in this gene result in exhibit an 80 to 90% reduction in 1,3-beta-glucan synthase specific activity. gns1 mutant strains display a pleiotropic phenotype including resistance to a pneumocandin B0 analog (L-733,560), slow growth, and mating and sporulation defects. The gns1-1 mutation was genetically mapped to within 1.35 centimorgans from the MAT locus on chromosome III. The wild-type GNS1 gene was isolated by complementing the pneumocandin resistance phenotype of the gns1-1 mutation and by hybridization with a chromosome III-derived sequence being used as a probe. The nucleotide sequence of GNS1 was determined and compared with the homologous region of the chromosome. The genetic and nucleotide sequence analyzes revealed that GNS1 and the open reading frame, YCR34 [S. Oliver, Q. van der Aart, M. Agostoni-Carbone, and the Chromosome III Sequencing Group, Nature (London) 357:38-46, 1992], represent identical loci in the genome. Cells deleted for GNS1 are viable but exhibit slow growth as well as the pleiotropic phenotype of the gns1 mutants. The putative protein product is predicted to be an integral membrane protein with five transmembrane helices displaying an exoplasmic orientation for the N terminus and a cytoplasmic orientation for the C terminus. This protein may be a subunit of 1,3-beta-glucan synthase.

更新日期：2019-11-01

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