MglA的功能，MglA是一种22千达尔顿的蛋白质，对于在粘液球菌中滑动至关重要。,Journal of Bacteriology - X-MOL

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MglA的功能，MglA是一种22千达尔顿的蛋白质，对于在粘液球菌中滑动至关重要。
Journal of Bacteriology ( IF 2.7 ) Pub Date : 1991-12-01 , DOI: 10.1128/jb.173.23.7615-7624.1991
P Hartzell ₁ , D Kaiser

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黄色粘球菌中mglA基因的单突变使细胞无法滑动。mglA菌株的独特之处在于，分离出的所有其他黄花不动杆菌菌株是分开的运动系统基因中至少两个独立突变的结果。构建了trpE或lacZ与mglA的翻译融合物，并将所得融合多肽用于生成抗体。纯化了对MglA蛋白特异的抗体。通过细胞提取物的分级分离和全细胞薄片的电子显微镜观察，发现带有抗体标签的MglA定位于黄连线虫细胞质。测试的五个mglA错义突变体中有四个未能在全细胞提取物中产生可检测水平的MglA抗原。非运动型双突变体（AS-），它们在系统A的一个基因中有一个突变，在系统S的一个基因中有一个突变，它们的表型与无效的mglA突变体相同，但产生的野生型水平的MglA蛋白。MglA蛋白在所有经测试的粘菌菌株中均保守。MglA蛋白的氨基酸序列包括三个GDP / GTP结合蛋白特征的序列基序。根据其遗传特性，细胞内位置和氨基酸序列，有人认为MglA蛋白是导致细胞运动的功能序列的调节剂。

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Function of MglA, a 22-kilodalton protein essential for gliding in Myxococcus xanthus.

Single mutations in the mglA gene in Myxococcus xanthus render cells incapable of gliding. The mglA strains are unique in that all other nonmotile strains of M. xanthus isolated are the result of at least two independent mutations in separate motility system genes. Translational fusions of trpE, or of lacZ, to mglA were constructed, and the resulting fusion polypeptides were used to generate antibodies. Antibodies specific to MglA protein were purified. Antibody-tagged MglA was found localized to the cytoplasm of M. xanthus cells both by fractionation of cell extracts and by electron microscopy of thin sections of whole cells. Four of the five mglA missense mutants tested failed to produce detectable levels of the MglA antigen in whole cell extracts. Nonmotile double mutants (A-S-), which have one mutation in a gene of system A and one mutation in a gene of system S, have the same phenotype as null mglA mutants but produce wild-type levels of MglA protein. MglA protein is conserved in all strains of myxobacteria tested. The amino acid sequence of MglA protein includes three sequence motifs characteristic of GDP/GTP-binding proteins. On the basis of its genetic properties, intracellular location, and amino acid sequence, it is argued that MglA protein is a regulator in the sequence of functions leading to cell movement.

更新日期：2019-11-01

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