运动发酵单胞菌glf，zwf，edd和glk基因形成操纵子：启动子的定位和在调控区的保守序列的鉴定。,Journal of Bacteriology

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运动发酵单胞菌glf，zwf，edd和glk基因形成操纵子：启动子的定位和在调控区的保守序列的鉴定。
Journal of Bacteriology ( IF 2.7 ) Pub Date : 1992-05-01 , DOI: 10.1128/jb.174.9.2816-2823.1992
W O Barnell ₁ , J Liu , T L Hesman , M C O'Neill , T Conway

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编码葡萄糖促进扩散转运蛋白（glf），葡萄糖6-磷酸脱氢酶（zwf），6-磷酸葡萄糖酸脱水酶（edd）和葡萄糖激酶（glk）的运动发酵单胞菌基因被聚集在基因组上。此处提供的数据牢固地确定了glf，zwf，edd和glk基因按此顺序形成操纵子。操纵子的四个基因在6.14-kb mRNA上共转录。通过引物延伸和核酸酶S1保护分析来定位多顺反子信息的转录起始位点。glf操纵子启动子区域与其他高度表达的运动发酵单胞菌启动子显示出显着同源性，但与其他细菌的共有启动子没有显着同源性。高度表达的运动发酵单胞菌启动子集包含两个独立的重叠相对于转录起始位点从大约bp -100延伸至+15的保守序列。显示glf操纵子的表达受到碳源依赖性调节的影响。在果糖上生长的细胞中，mRNA水平比在葡萄糖上生长的细胞高三倍。当细胞在不同碳源上生长时，这种增加既不是差异mRNA加工的结果，也不是差异转录稳定性的结果。降解6.14-kb glf操纵子mRNA是双相的，在果糖生长的细胞中最初的半衰期为11.5分钟，在葡萄糖生长的细胞中为12.0分钟。因此，果糖生长的细胞中glf操纵子mRNA的较高水平是转录速率增加的结果。讨论了在果糖上生长的细胞中增加glf表达的重要性。

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The Zymomonas mobilis glf, zwf, edd, and glk genes form an operon: localization of the promoter and identification of a conserved sequence in the regulatory region.

The Zymomonas mobilis genes that encode the glucose-facilitated diffusion transporter (glf), glucose-6-phosphate dehydrogenase (zwf), 6-phosphogluconate dehydratase (edd), and glucokinase (glk) are clustered on the genome. The data presented here firmly establish that the glf, zwf, edd, and glk genes form an operon, in that order. The four genes of the operon are cotranscribed on a 6.14-kb mRNA. The site of transcriptional initiation for the polycistronic message was mapped by primer extension and nuclease S1 protection analysis. The glf operon promoter region showed significant homology to other highly expressed Z. mobilis promoters, but not to consensus promoters from other bacteria. The highly expressed Z. mobilis promoter set contains two independent, overlapping, conserved sequences that extend from approximately bp -100 to +15 with respect to the transcriptional start sites. Expression of the glf operon was shown to be subject to carbon source-dependent regulation. The mRNA level was threefold higher in cells grown on fructose than in cells grown on glucose. This increase was not the result of differential mRNA processing when cells were grown on the different carbon sources, nor was it the result of differential transcript stability. Degradation of the 6.14-kb glf operon mRNA was biphasic, with initial half-lives of 11.5 min in fructose-grown cells and 12.0 min in glucose-grown cells. Thus, the higher level of glf operon mRNA in fructose-grown cells is the result of an increased rate of transcription. The importance of increasing glf expression in cells growing on fructose is discussed.

更新日期：2019-11-01

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