2-O-α-d-glucopyranosyl-l-ascorbic acid (AA-2G) is one of the most important l-ascorbic acid derivatives because of its resistance to reduction and oxidation and its easy degradation by α-glucosidase to release l-ascorbic acid and glucose. Thus, AA-2G has commercial uses in food, medicines and cosmetics. This article presents a review of recent studies on the enzymatic production of AA-2G using cyclodextrin glycosyltransferase. Reaction mechanisms with different donor substrates are discussed. Protein engineering, physical and biological studies of cyclodextrin glycosyltransferase are introduced from the viewpoint of effective AA-2G production. Future prospects for the production of AA-2G using cyclodextrin glycosyltransferase are reviewed.

2-O-α- d-吡喃葡萄糖基-1-抗坏血酸（AA-2G）是最重要的1-抗坏血酸衍生物之一，因为它具有抗还原和氧化作用，并且易于被α-葡萄糖苷酶降解以释放l-抗坏血酸和葡萄糖。因此，AA-2G在食品，药品和化妆品中具有商业用途。本文介绍了使用环糊精糖基转移酶酶促生产AA-2G的最新研究综述。讨论了不同供体底物的反应机理。从有效生产AA-2G的角度介绍了蛋白质工程，环糊精糖基转移酶的物理和生物学研究。综述了使用环糊精糖基转移酶生产AA-2G的未来前景。