受体活性修饰蛋白1过度表达对小鼠脑毛细血管内皮细胞血管生成的影响。,Journal of Cellular Biochemistry

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受体活性修饰蛋白1过度表达对小鼠脑毛细血管内皮细胞血管生成的影响。
Journal of Cellular Biochemistry ( IF 3.0 ) Pub Date : 2018-12-16 , DOI: 10.1002/jcb.28292
Qin Zhang _{1,

2} , Yanjun Guo _{1,

2} , Huilu Chen _{1,

2} , Yixuan Jiang _{1,

2} , Haiyang Tang _{1,

2} , Ping Gong _{1,

2} , Lin Xiang _{1,

2}

Affiliation

受体活性修饰蛋白1（RAMP1）在心脏和血管中高表达，表明它可能与血管系统有关。然而，RAMP1对血管生成的影响和这一过程的内在机制尚不清楚。在这里，我们验证了RAMP1是小鼠脑毛细血管内皮细胞系（bEnd.3）中血管生成的关键调节剂。我们首先构建了RAMP1过表达慢病毒载体系统，并稳定转染了bEnd.3细胞。我们进一步表明，未经外源降钙素基因相关肽（CGRP）处理，RAMP1的过度表达可能导致Matrigel中bEnd.3迁移和毛细管形成。同时，RAMP1过表达对增殖的影响很小。更重要的是，在过表达RAMP1的细胞中，血管内皮生长因子（VEGF）和CGRP的表达水平没有显着高于对照细胞（P> 0.05），这表明RAMP1不能通过上调bEnd.3细胞中的VEGF或CGRP的表达发挥作用。令人惊讶的是，RAMP1转染增加了肾上腺髓质素2（AM2）的表达水平（P <0.05）。综上所述，这些数据有助于更好地了解RAMP1在血管生成中的分子机制。

"点击查看英文标题和摘要"

The influence of receptor activity-modifying protein-1 overexpression on angiogenesis in mouse brain capillary endothelial cells.

Receptor activity-modifying protein-1 (RAMP1) is highly expressed in the heart and vasculature, indicating that it might be related to the vascular system. However, the effects of RAMP1 on angiogenesis and the intrinsic mechanisms underlying this process remain unclear. Here, we verified that RAMP1 is a critical regulator of angiogenesis in a mouse brain capillary endothelial cell line (bEnd.3). We first constructed a RAMP1 overexpression lentiviral vector system and stably transfected bEnd.3 cells. We further showed that RAMP1 overexpression could lead to bEnd.3 migration and capillary tube formation in Matrigel without exogenous calcitonin gene-related peptide (CGRP) treatment. At the same time, RAMP1 overexpression had little effect on proliferation. More importantly, vascular endothelial growth factor (VEGF) and CGRP expression levels were not significantly higher in RAMP1-overexpressing cells than in control cells (P > 0.05), indicating that RAMP1 did not function through upregulating VEGF or CGRP expression in bEnd.3 cells. Strikingly, RAMP1 transfection increased adrenomedullin 2 (AM2) expression levels ( P < 0.05). Taken together, these data contribute to a better understanding of the molecular mechanisms of RAMP1 in angiogenesis.

更新日期：2019-11-01

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