Chronic inflammation of the myelin sheath is the crucial event behind the progression of multiple sclerosis (MS). Bacoside-A is one of the major constituents obtained from Bacopa monerii (L.) Wettst., and possess neuroprotective as well as anti-inflammatory actions. The current study explores the effect of Bacoside-A in acute and chronic models of Experimental Autoimmune Encephalomyelitis (EAE). The results indicate that the Bacoside-A treated mice produced a significant reduction in disease score compared to disease control in both models. The treatment with Bacoside-A downregulated the inflammatory cytokines (IL-6, IL-17a, and TNFα) and inflammatory chemokine CCL-5 in EAE mice. On the other hand, Bacoside-A treated mice showed a nonsignificant effect on promoting the expressions of NCAM, BDNF1, and FOXP3 in acute and chronic models of EAE. Histopathological analysis revealed that the Bacoside-A treated mice at a dose of 10 mg/kg exhibited a significant reduction in cellular infiltrations, cellular changes, and demyelination in cerebral tissues, but unable to protect at a higher dose in both models. In conclusion, Bacoside-A can able to inhibit the progression of EAE may be by the inhibition of inflammatory cytokines and chemokine evolved during active EAE.

中文翻译：

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Bacoside-A在实验性自身免疫性脑脊髓炎中抑制炎症细胞因子和趋化因子。

髓鞘的慢性炎症是多发性硬化症（MS）进展的关键事件。Bacoside-A是从Bacopa monerii（L.）Wettst。获得的主要成分之一，并具有神经保护和抗炎作用。当前的研究探讨了Bacoside-A在实验性自身免疫性脑脊髓炎（EAE）急性和慢性模型中的作用。结果表明，与两种模型中的疾病对照相比，用Bacoside-A处理的小鼠的疾病评分显着降低。Bacoside-A的处理下调了EAE小鼠的炎性细胞因子（IL-6，IL-17a和TNFα）和炎性趋化因子CCL-5。另一方面，用Bacoside-A处理的小鼠在EAE的急性和慢性模型中，对促进NCAM，BDNF1和FOXP3的表达没有显着影响。组织病理学分析显示，以10 mg / kg的剂量用Bacoside-A处理的小鼠在脑组织中的细胞浸润，细胞变化和脱髓鞘作用显着降低，但在两种模型中均不能以更高的剂量提供保护。总之，Bacoside-A能够抑制EAE的进程可能是通过抑制活性EAE期间产生的炎症细胞因子和趋化因子而实现的。