具有抗糖化剂的pH响应水凝胶，可调节实验性牙周炎。,Journal of Periodontology

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具有抗糖化剂的pH响应水凝胶，可调节实验性牙周炎。
Journal of Periodontology ( IF 4.2 ) Pub Date : 2016-02-19 , DOI: 10.1902/jop.2016.150542
Min‐Chen Yu , Chih‐Yeun Chang , Yi‐Chi Chao , Yi‐Han Jheng , Connie Yang , Ning Lee , Shan‐Huey Yu , Xin‐Hong Yu , Dean‐Mo Liu , Po‐Chun Chang

背景技术刺激响应装置已经出现为用于局部药物递送的新方法。这项研究调查了一种新型的基于壳聚糖，pH响应的水凝胶的可行性，该水凝胶负载了N-苯甲酰噻唑溴化物（PTB），可裂解细胞外基质上高级糖基化终产物的交联。方法制备了载有PTB的壳聚糖基水凝胶，并在pH 5.5至7.4范围内评估了其体外释放特性。用BALB / cJ小鼠和Sprague-Dawley大鼠分别评估在牙周炎的诱导和恢复阶段的效果，并将每个阶段的动物分为四组：1）无牙周炎诱导；2）结扎诱导的实验性牙周炎（PR组）；3）实验性牙周炎加无PTB的水凝胶（PH组）；4）实验性牙周炎加水凝胶配PTB（PP组）。通过牙周骨水平（PBL）丢失的显微计算机断层成像以及炎性细胞浸润和胶原蛋白密度的组织形态测定法评估疗效。结果PTB在pH 5.5至6.5时释放较快，而在pH 7.4时释放较慢。在诱导阶段，PP组相对于PR组，PBL和炎性细胞浸润明显减少，而PH组则明显减少了胶原基质的损失。在恢复阶段，在移丝后第4天和第14天，PP组相对于PR和PH组，PBL和炎性细胞浸润明显减少，并且PP组的胶原蛋白沉积明显增加。结论基于壳聚糖的，

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pH-Responsive Hydrogel With an Anti-Glycation Agent for Modulating Experimental Periodontitis.

BACKGROUND Stimulus-responsive devices have emerged as a novel approach for local drug delivery. This study investigates the feasibility of a novel chitosan-based, pH-responsive hydrogel loaded with N-phenacylthiazolium bromide (PTB), which cleaves the crosslinks of advanced glycation end products on the extracellular matrix. METHODS A chitosan-based hydrogel loaded with PTB was fabricated, and the in vitro release profile was evaluated within pH 5.5 to 7.4. BALB/cJ mice and Sprague-Dawley rats were used to evaluate the effects during the induction and recovery phases of periodontitis, respectively, and animals in each phase were divided into four groups: 1) no periodontitis induction; 2) ligature-induced experimental periodontitis (group PR); 3) experimental periodontitis plus hydrogel without PTB (group PH); and 4) experimental periodontitis plus hydrogel with PTB (group PP). The therapeutic effects were evaluated by microcomputed tomographic imaging of periodontal bone level (PBL) loss and histomorphometry for inflammatory cell infiltration and collagen density. RESULTS PTB was released faster at pH 5.5 to 6.5 and consistently slower at pH 7.4. In the induction phase, PBL and inflammatory cell infiltration were significantly reduced in group PP relative to group PR, and the loss of collagen matrix was significantly reduced relative to that observed in group PH. In the recovery phase, PBL and inflammatory cell infiltration were significantly reduced, and significantly greater collagen deposition was noted in group PP relative to groups PR and PH at 4 and 14 days after silk removal. CONCLUSION Chitosan-based, pH-responsive hydrogels loaded with PTB can retard the initiation of and facilitate the recovery from experimental periodontitis.

更新日期：2019-11-01

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