A sensitive and accurate LC-MS/MS method was established for quantifying bisabolangelone in rat plasma and tissues. Bisabolangelone was isolated and purified from Angelicae Pubescentis Radix. The pharmacokinetic and tissue distribution of bisabolangelone after administration to rat was performed by LC-MS/MS. Separation was carried out on a C8 (4.6 × 100 mm, 1.8 μm) column. The MS/MS transitions of bisabolangelone and tussilagone (internal standard) were set at m/z 249.1 → 109.1 and m/z 391.4 → 217.4, respectively. The lower limit of quantification in plasma and other tissues ranged from 1 to 4 ng/mL. The biosamples were prepared using protein precipitation method with acetonitrile. The recovery was >92%. The results showed that values of maximum concentrations and area under the curve depended linearly on the studied doses (2.5, 5 and 7.5 mg/kg body weight). The other ingredients in Angelicae Pubescentis Radix extract possibly reduce the absorption of bisabolangelone in rat. Tissue distribution revealed that bisabolangelone was widely distributed in vivo. The highest and lowest concentrations of bisabolangelone were found in the stomach and in the brain, respectively. It was concluded that the newly established HPLC-MS/MS method was suitable to describe the pharmacokinetic characteristics of bisabolangelone in rat after administration.

中文翻译：

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当归在大鼠中的药代动力学和组织分布的LC-MS / MS研究。

建立了灵敏，准确的LC-MS / MS方法，用于定量大鼠血浆和组织中的双硼苯醌。从当归中分离和纯化双雪佛兰酮。通过LC-MS / MS对大鼠给药后双硼苯醌的药代动力学和组织分布。在C8（4.6×100 mm，1.8μm）色谱柱上进行分离。双abolangelone和tussilagone（内标）的MS / MS转换分别设置为m / z 249.1→109.1和m / z 391.4→217.4。血浆和其他组织的定量下限为1-4 ng / mL。使用蛋白质沉淀法和乙腈制备生物样品。回收率> 92％。结果表明，最大浓度和曲线下面积的值与研究剂量（2.5、5和7）线性相关。5 mg / kg体重）。当归当归提取物中的其他成分可能会减少双abolangelone在大鼠中的吸收。组织分布表明，双abolangelone在体内广泛分布。在胃和脑中分别发现了最高和最低浓度的双abolangelone。结论是，新建立的HPLC-MS / MS方法适用于描述双硼苯醌在大鼠给药后的药代动力学特征。