在与尿道相关的铜绿假单胞菌分离株中毒力因子的适应和多样化。,Journal of Applied Microbiology

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在与尿道相关的铜绿假单胞菌分离株中毒力因子的适应和多样化。
Journal of Applied Microbiology ( IF 3.2 ) Pub Date : 2018-10-30 , DOI: 10.1111/jam.14143
C Vipin _{1,

2} , M Mujeeburahiman ₂ , A B Arun ₁ , P Ashwini ₁ , S V Mangesh ₁ , P D Rekha ₁

Affiliation

目的本研究的目的是调查从单个中心的导管相关性尿路感染（CAUTI）中分离出的铜绿假单胞菌的抗生素敏感性，生物膜形成和毒力的自然变化。方法和结果获得知情同意后，从CAUTI患者中分离出铜绿假单胞菌菌株。通过常规生化方法和16S rRNA基因测序鉴定这些分离株。使用标准方案测量抗生素敏感性和毒力因子。结晶紫染色，扫描电子显微镜和共聚焦激光扫描显微镜用于生物膜研究。使用人胚肾细胞（HEK 293T）在体外研究了菌株诱导细胞裂解的感染性程度。毒力因子之间的关联，统计分析菌株之间的生物膜形成和抗生素抗性。在2016-2017年期间收治的1266例患者中，报告了98例CAUTI病例，其中18.36％（n = 18）是由铜绿假单胞菌感染引起的。抗菌谱显示94.4％的分离株对多种抗生素有抗药性，而73.7％的对碳青霉烯有抗药性。所有分离株在不同材料表面上形成生物膜，强度不同（OD580≥0·20-1·11）。与聚苯乙烯表面相比，有机硅-乳胶材料上的生物膜强度明显更高（P> 0·05）。所有菌株均具有高毒性，能够以69·35至100％的速率杀死HEK 293T细胞，并且对健康人血清的敏感性非常低。结论铜绿假单胞菌临床菌株对抗生素的敏感性以及毒力因子与生物膜形成之间的关联显示出复杂的自然多样性。该研究的意义和影响该研究证明了与导管相关的铜绿假单胞菌菌株之间毒力因子，生物膜形成和宿主-病原体相互作用的自然多样性和适应性。该研究的发现敦促发展针对这些耐多药病原体的个体化药物策略。

"点击查看英文标题和摘要"

Adaptation and diversification in virulence factors among urinary catheter-associated Pseudomonas aeruginosa isolates.

AIM The aim of this study was to investigate the natural variation in the antibiotic sensitivity, biofilm formation and virulence among Pseudomonas aeruginosa isolated from the catheter-associated urinary tract infection (CAUTI) from a single centre. METHODS AND RESULTS Pseudomonas aeruginosa strains were isolated from the patients with CAUTI after obtaining informed consent. These isolates were identified by routine biochemical methods and 16S rRNA gene sequencing. Antibiotic sensitivity and virulence factors were measured using standard protocols. Crystal violet staining, scanning electron microscopy and confocal laser scanning microscopy were used for the biofilm studies. The extent of infectivity of the strains to induce cell lysis was studied in vitro using the Human Embryonic Kidney cells (HEK 293T). Association between virulence factors, biofilm formation and antibiotic resistance among the strains was analysed statistically. Among the 1266 patients admitted during the 2016-2017 period, 98 cases of CAUTI were reported and 18·36% (n = 18) was due to P. aeruginosa infection. Antibiogram showed that 94·4% of isolates were resistant to multiple antibiotics and 73·7% were carbapenem-resistant. All the isolates formed biofilm on different material surfaces with varying intensity (OD580 ≥0·20-1·11). The biofilm intensity on silicone-latex material was significantly higher compared to the polystyrene surface (P > 0·05). All the strains were highly virulent and able to cause cell killing of HEK 293T cells with a rate ranging from 69·35 to 100% and showed very low sensitivity to healthy human serum. CONCLUSIONS Antibiotic sensitivity and association between the virulence factors and biofilm formation in the P. aeruginosa clinical strains showed complex natural diversity. SIGNIFICANCE AND IMPACT OF THE STUDY This study demonstrates the natural diversity and adaptation in virulence factors, biofilm formation and host-pathogen interaction among catheter-associated P. aeruginosa strains. The findings from the study urge for developing individualized drug strategy for targeting these multidrug-resistant pathogens.

更新日期：2019-11-01

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