Sagittatoside B, a rare secondary flavonol glycoside in Epimedii Folium, has much better in vivo bioactivities than its original glycoside epimedin B. Its preparation methods, such as acidic hydrolysis, are of low efficiency, and byproducts are generated. The objective of this study was to establish a novel catalysis system for convenient preparation of this compound based on recyclable and integrated biphase enzymatic hydrolysis. β-glucanase was selected from five commercial enzymes based on the best catalysis performance. After optimization of the conditions, the biphase system was constructed with propyl acetate and HAc-NaAc buffer (pH 4.5) (1:1, v/v) containing β-glucanase/epimedin B (1:2, w/w), and the hydrolysis was performed at 60 °C for 1 h. Consequently, epimedin B was completely hydrolyzed to sagittatoside B, and 95.7% of the product was transferred into the organic phase. Moreover, a high conversion ratio of 94.0% was achieved, even after the enzyme solution was used for six cycles. Additionally, the procedure was much simplified compared with conventional enzymatic hydrolysis. The newly proposed strategy is an efficient and promising approach for the preparation of sagittatoside B in industrial applications.

中文翻译：

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通过可回收和整合的双相酶水解方便地制备一种罕见的生物活性次级黄酮醇糖苷 sagittatoside B

淫羊藿苷B是一种罕见的淫羊藿次生黄酮苷，其体内生物活性远优于其原配苷淫羊藿苷B，其酸性水解等制备方法效率低下，且会产生副产物。本研究的目的是建立一种基于可回收和集成双相酶水解方便制备该化合物的新型催化体系。β-葡聚糖酶是根据最佳催化性能从五种商业酶中选出的。优化条件后，用乙酸丙酯和含有 β-葡聚糖酶/淫羊藿苷 B (1:2, w/w) 的 HAc-NaAc 缓冲液 (pH 4.5) (1:1, v/v) 构建双相系统，并水解在 60 °C 下进行 1 h。因此，淫羊藿苷 B 被完全水解为 sagittatoside B 和 95。7%的产物被转移到有机相中。此外，即使在酶溶液使用六个循环后，也实现了 94.0% 的高转化率。此外，与传统的酶水解相比，该过程大大简化。新提出的策略是一种有效且有前景的方法，用于在工业应用中制备矢状糖苷 B。