玉米中的甘油3-磷酸脱氢酶（GPDH）基因家族：鉴定，亚细胞定位和对非生物胁迫的转录反应。,PLOS ONE

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玉米中的甘油3-磷酸脱氢酶（GPDH）基因家族：鉴定，亚细胞定位和对非生物胁迫的转录反应。
PLOS ONE ( IF 2.9 ) Pub Date : 2018-07-11 , DOI: 10.1371/journal.pone.0200357
Ying Zhao ₁ , Xin Li ₂ , Feng Wang ₁ , Xunchao Zhao ₁ , Yuqiao Gao ₁ , Changjiang Zhao ₁ , Lin He ₁ , Zuotong Li ₁ , Jingyu Xu ₁

Affiliation

3-磷酸甘油脱氢酶（GPDH）催化3-磷酸甘油的形成，并在甘油脂代谢和响应不同物种的各种胁迫中起重要作用。在这项研究中，通过彻底搜索玉米基因组获得了六个ZmGPDH基因，分别命名为ZmGPDH1-6。结构和进化分析表明，ZmGPDHs家族具有典型的保守结构域和与其他植物物种已知的GPDHs相似的蛋白质结构。ZmGPDHs根据其N端序列分为NAD +依赖性A型（ZmGPDH1-5）和FAD依赖性B型（ZmGPDH6）。将四个全长ZmGPDHs与GFP融合蛋白融合，并确定其亚细胞定位。ZmGPDH1和ZmGPDH3位于细胞质中，主要募集到内质网（ER）的表面，而ZmGPDH4和ZmGPDH5位于叶绿体中。ZmGPDHs在不同玉米组织中的转录分析表明，ZmGPDHs在根和早期发育种子中的转录物积累水平相对较高。此外，我们检查了玉米中六个GPDH基因在各种非生物胁迫（包括盐，干旱，碱和冷）下的转录应答，并观察到在渗透胁迫下ZmGPDHs的显着诱导。总之，这项工作将为破译GPDH在植物发育和非生物胁迫响应中的作用提供有用的信息。ZmGPDHs在不同玉米组织中的转录分析表明，ZmGPDHs在根和早期发育种子中的转录物积累水平相对较高。此外，我们检查了玉米中六个GPDH基因在各种非生物胁迫（包括盐，干旱，碱和冷）下的转录应答，并观察到在渗透胁迫下ZmGPDHs的显着诱导。总之，这项工作将为破译GPDH在植物发育和非生物胁迫响应中的作用提供有用的信息。ZmGPDHs在不同玉米组织中的转录分析表明，ZmGPDHs在根和早期发育种子中的转录物积累水平相对较高。此外，我们检查了玉米中六个GPDH基因在各种非生物胁迫（包括盐，干旱，碱和冷）下的转录应答，并观察到在渗透胁迫下ZmGPDHs的显着诱导。总之，这项工作将为破译GPDH在植物发育和非生物胁迫响应中的作用提供有用的信息。并观察到在渗透胁迫下ZmGPDHs的显着诱导。总之，这项工作将为破译GPDH在植物发育和非生物胁迫响应中的作用提供有用的信息。并观察到在渗透胁迫下ZmGPDHs的显着诱导。总之，这项工作将为破译GPDH在植物发育和非生物胁迫响应中的作用提供有用的信息。

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Glycerol-3-phosphate dehydrogenase (GPDH) gene family in Zea mays L.: Identification, subcellular localization, and transcriptional responses to abiotic stresses.

Glycerol-3-phosphate dehydrogenase (GPDH) catalyzes the formation of glycerol-3-phosphate, and plays an essential role in glycerolipid metabolism and in response to various stresses in different species. In this study, six ZmGPDH genes were obtained by a thorough search against maize genome, and designated as ZmGPDH1-6, respectively. The structural and evolutionary analyses showed that the ZmGPDHs family had typical conserved domains and similar protein structures as the known GPDHs from other plant species. ZmGPDHs were divided into NAD+-dependent type A form (ZmGPDH1-5) and FAD-dependent type B form (ZmGPDH6) based on their N-terminal sequences. Four full length ZmGPDHs were fused with GFP fusion proteins, and their subcellular localization was determined. ZmGPDH1 and ZmGPDH3 were located to the cytosol and mainly recruited to the surface of endoplasmic reticulum (ER), whereas ZmGPDH4 and ZmGPDH5 were located in the chloroplast. The transcriptional analysis of the ZmGPDHs in different maize tissues revealed relatively high level of transcripts accumulation of ZmGPDHs in roots and early stage developing seeds. Furthermore, we examined the transcriptional responses of the six GPDH genes in maize under various abiotic stresses, including salt, drought, alkali and cold, and significant induction of ZmGPDHs under osmotic stresses was observed. Together, this work will provide useful information for deciphering the roles of GPDHs in plant development and abiotic stress responses.

更新日期：2019-11-01

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