2-Deoxy-D-ribose-5-phosphate aldolase (DERA) is a class I aldolase that offers access to several building blocks for organic synthesis. It catalyzes the stereoselective C-C bond formation between acetaldehyde and numerous other aldehydes. However, the practical application of DERA as a biocatalyst is limited by its poor tolerance towards industrially relevant concentrations of aldehydes, in particular acetaldehyde. Therefore, the development of proper experimental conditions, including protein engineering and/or immobilization on appropriate supports, is required. The present review is aimed to provide a brief overview of DERA, its history, and progress made in understanding the functioning of the enzyme. Furthermore, the current understanding regarding aldehyde resistance of DERA and the various optimizations carried out to modify this property are discussed.

中文翻译：

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2-脱氧-D-核糖-5-磷酸醛缩酶 (DERA)：应用和修饰。


2-脱氧-D-核糖-5-磷酸醛缩酶 (DERA) 是一种 I 类醛缩酶，可提供多种有机合成结构单元。它催化乙醛和许多其他醛之间立体选择性 CC 键的形成。然而，DERA 作为生物催化剂的实际应用因其对工业相关浓度的醛（特别是乙醛）的耐受性差而受到限制。因此，需要开发适当的实验条件，包括蛋白质工程和/或固定在适当的支持物上。本综述旨在简要概述 DERA、其历史以及在了解酶功能方面取得的进展。此外，还讨论了目前对 DERA 抗醛性的理解以及为改变该特性而进行的各种优化。