<br>壳聚糖纳米层载顺铂脂质纳米颗粒可增强宫颈癌的抗癌功效。,Nanoscale Research Letters

当前位置： X-MOL 学术 › Nanoscale Res. Lett. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

壳聚糖纳米层载顺铂脂质纳米颗粒可增强宫颈癌的抗癌功效。
Nanoscale Research Letters ( IF 5.5 ) Pub Date : 2016-11-25 , DOI: 10.1186/s11671-016-1698-9
Jing-Yi Wang ₁ , Yu Wang ₂ , Xia Meng ₁

Affiliation

在这项研究中，成功配制了负载顺铂（CDDP）的壳聚糖包被的固体脂质纳米粒（SLN）来治疗HeLa宫颈癌。制剂纳米颗粒是纳米级的，在生理条件下表现出药物的受控释放。空白纳米粒子表现出优异的生物相容性，表明其适合癌症靶向。 MTT 测定表明，SLN 中掺入 CDDP 显着增加了癌细胞死亡。重要的是，即使在低浓度下，负载 CDDP 的壳聚糖包被的 SLN (CChSLN) 也能显着 (P < 0.05) 降低癌细胞的活力。与 CSLN 相比，CChSLN 较高的细胞毒性潜力归因于更高的细胞摄取以及持续的药物释放方式。与细胞毒性测定一致，CChSLN 显示最低 IC50 值为 0.6125 μg/ml，而 CSLN 为 1.156 μg/ml。与CSLN和CDDP相比，CChSLN在癌细胞中表现出显着更高的凋亡，这归因于纳米载体更好的内化和抗癌药物在细胞内环境中的受控释放。我们的研究结果表明，这种新制剂可能成为治疗宫颈癌的有前途的替代方案。这些发现鼓励我们继续我们的研究，对动物模型中的细胞反应进行更广泛的实时研究。

"点击查看英文标题和摘要"

Chitosan Nanolayered Cisplatin-Loaded Lipid Nanoparticles for Enhanced Anticancer Efficacy in Cervical Cancer.

In this study, cisplatin (CDDP)-loaded chitosan-coated solid lipid nanoparticles (SLN) was successfully formulated to treat HeLa cervical carcinoma. The formulation nanoparticles were nanosized and exhibited a controlled release of drug in physiological conditions. The blank nanoparticles exhibited an excellent biocompatibility profile indicating its suitability for cancer targeting. The incorporation of CDDP in SLN remarkably increased the cancer cell death as evident from the MTT assay. Importantly, CDDP-loaded chitosan-coated SLN (CChSLN) significantly (P < 0.05) decreased the viability of cancer cells even at low concentration. The higher cytotoxicity potential of CChSLN was attributed to the higher cellular uptake as well as the sustained drug release manner in comparison with CSLN. Consistent with the cytotoxicity assay, CChSLN showed the lowest IC50 value of 0.6125 μg/ml while CSLN presented 1.156 μg/ml. CChSLN showed a significantly higher apoptosis in cancer cells compared to that of CSLN and CDDP, which is attributed to the better internalization of nanocarriers and controlled release of anticancer drugs in the intracellular environment. Our findings suggest that this new formulation could be a promising alternative for the treatment of cervical cancers. These findings are encouraging us to continue our research, with a more extended investigation of cellular response in real time and in animal models.

更新日期：2019-11-01

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南