除草剂flamprop-M-methyl具有新的抗微管作用机理。,Pest Management Science

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除草剂flamprop-M-methyl具有新的抗微管作用机理。
Pest Management Science ( IF 3.8 ) Pub Date : 2008-06-14 , DOI: 10.1002/ps.1618
Stefan Tresch ₁ , Ricarda Niggeweg , Klaus Grossmann

Affiliation

背景技术研究了火焰丙酯-M-甲基[甲基N-苯甲酰基-N-（3-氯-4-氟苯基）-D-丙氨酸盐]的除草作用方式。结果为了进行初步表征，使用了一系列生物测定方法，表明其作用方式与有丝分裂破坏性除草剂相似。细胞化学荧光研究包括针对聚合微管蛋白的单克隆抗体，用于阐明对玉米根部有丝分裂和微管组装的影响。当用50 microM的flamprop-M-methyl根处理幼苗时，分生组织的根尖细胞的细胞分裂活性在4小时内停止。该化合物严重干扰纺锤体和成膜细胞微管的取向，导致纺锤体和成膜细胞结构缺陷。皮质微管仅受到轻微影响。在后期后期和早期末期细胞中，成纤维细胞微管被分散成多个阵列，从而妨碍了细胞分裂中规则的细胞板沉积。发现纺锤体设备的微管附着在染色体的动植物上，但未显示出与细胞相对两端的微管组织中心区域相关的规则组织。由于纺锤体组织的这种丧失，染色体保持在前中期或中期的浓缩状态。与已知的微管破坏剂除草剂不同，flamprop-M-甲基及其生物活性代谢物flamprop在体外以50 microM的浓度不抑制大豆微管蛋白聚合成微管。相反，大豆植物对这些化合物敏感。

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The herbicide flamprop-M-methyl has a new antimicrotubule mechanism of action.

BACKGROUND The herbicidal mode of action of flamprop-M-methyl [methyl N-benzoyl-N-(3-chloro-4-fluorophenyl)-D-alaninate] was investigated. RESULTS For initial characterization, a series of bioassays was used, which indicated a mode of action similar to that of mitotic disrupter herbicides. Cytochemical fluorescence studies, which included monoclonal antibodies against polymerized tubulin, were applied to elucidate effects on mitosis and microtubule assembly in maize roots. When seedlings were root treated with 50 microM of flamprop-M-methyl, cell division activity in meristematic root tip cells ceased within 4 h. The compound severely disturbed the orientation of spindle and phragmoblast microtubules, leading to defective spindle and phragmoblast structures. Cortical microtubules were only slightly affected. In late anaphase and early telophase cells, phragmoblast microtubules were disorganized in multiple arrays that hampered regular cell plate deposition in cytokinesis. Microtubules of the spindle apparatus were found attached to chromosomal kinetochores, but did not show regular organization associated with a zone of microtubule-organizing centres at the opposite ends of the cell. On account of this loss of spindle organization, chromosomes remained in a condensed state of prometaphase or metaphase. Unlike known microtubule disrupter herbicides, flamprop-M-methyl and its biologically active metabolite flamprop did not inhibit soybean tubulin polymerization to microtubules in vitro at 50 microM. In contrast, soybean plants responded sensitively to the compounds. CONCLUSION The results indicate that flamprop-M-methyl is a mitotic disrupter herbicide with a new antimicrotubule mechanism of action that affects orientation of spindle and phragmoblast microtubules, possibly by minus-end microtubule disassembly.

更新日期：2019-11-01

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