蓝莓花青素 malvidin-3-glucoside 和 malvidin-3-galactoside 在内皮细胞中的抗炎作用。,Molecules

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蓝莓花青素 malvidin-3-glucoside 和 malvidin-3-galactoside 在内皮细胞中的抗炎作用。
Molecules ( IF 4.2 ) Pub Date : 2014-08-21 , DOI: 10.3390/molecules190812827
Wu-Yang Huang ₁ , Ya-Mei Liu ₂ , Jian Wang ₁ , Xing-Na Wang ₁ , Chun-Yang Li ₁

Affiliation

蓝莓果实含有丰富的花青素（天然抗氧化剂），因此具有广泛的健康益处。本研究的目的是探讨蓝莓的两种主要花青素（锦葵素-3-葡萄糖苷和锦葵素-3-半乳糖苷）对内皮细胞炎症反应的抑制作用。这两种锦葵素苷可以抑制肿瘤坏死因子-α（TNF-α）诱导的单核细胞趋化蛋白-1（MCP-1）、细胞间粘附分子-1（ICAM-1）和血管细胞粘附分子-1（VCAM）的增加-1) 以浓度依赖性方式在蛋白质和mRNA水平上产生。1 μM浓度的Mv-3-glc可抑制上清液中增加的MCP-1、54.4% ICAM-1和44.7% VCAM-1蛋白35.9%，以及9.88% MCP-1和48.6% ICAM-1 mRNA 表达（p<0.05）。此外，它们还可以减少IκBα的降解（Mv-3-glc、Mv-3-gal及其混合物在50 μM浓度下的抑制率分别为84.8%、75.3%和43.2%，p<0.01 ）并阻断p65的核转位，这表明它们的抗炎机制是由核因子-κB（NF-κB）通路介导的。总体而言，锦葵素3-葡萄糖苷的抗炎作用优于锦葵素3-半乳糖苷。这些结果表明，蓝莓是抗炎花青素的良好来源，它可以成为开发预防多种疾病慢性炎症的营养保健品的有前途的分子。

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Anti-inflammatory effect of the blueberry anthocyanins malvidin-3-glucoside and malvidin-3-galactoside in endothelial cells.

Blueberry fruits have a wide range of health benefits because of their abundant anthocyanins, which are natural antioxidants. The purpose of this study was to investigate the inhibitory effect of blueberry's two main anthocyanins (malvidin-3-glucoside and malvidin-3-galactoside) on inflammatory response in endothelial cells. These two malvidin glycosides could inhibit tumor necrosis factor-alpha (TNF-α) induced increases of monocyte chemotactic protein-1 (MCP-1), intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) production both in the protein and mRNA levels in a concentration-dependent manner. Mv-3-glc at the concentration of 1 μM could inhibit 35.9% increased MCP-1, 54.4% ICAM-1, and 44.7% VCAM-1 protein in supernatant, as well as 9.88% MCP-1 and 48.6% ICAM-1 mRNA expression (p<0.05). In addition, they could decrease IκBα degradation (Mv-3-glc, Mv-3-gal, and their mixture at the concentration of 50 μM had the inhibition rate of 84.8%, 75.3%, and 43.2%, respectively, p<0.01) and block the nuclear translocation of p65, which suggested their anti-inflammation mechanism was mediated by the nuclear factor-kappa B (NF-κB) pathway. In general malvidin-3-glucoside had better anti-inflammatory effect than malvidin-3-galactoside. These results indicated that blueberry is good resource of anti-inflammatory anthocyanins, which can be promising molecules for the development of nutraceuticals to prevent chronic inflammation in many diseases.

更新日期：2019-11-01

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