细菌生物合成精氨酸脱羧酶的结构。,Acta Crystallographica Section F

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细菌生物合成精氨酸脱羧酶的结构。
Acta Crystallographica Section F ( IF 1.1 ) Pub Date : 2010-12-07 , DOI: 10.1107/s1744309110040649
Farhad Forouhar ₁ , Scott Lew , Jayaraman Seetharaman , Rong Xiao , Thomas B Acton , Gaetano T Montelione , Liang Tong

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生物合成精氨酸脱羧酶 (ADC；也称为 SpeA) 在细菌和植物中从精氨酸生物合成多胺中起着重要作用。SpeA 是一种 5'-磷酸吡哆醛 (PLP) 依赖性酶，与其他几种 PLP 依赖性脱羧酶具有弱序列同源性。在这里，来自空肠弯曲杆菌的 PLP 结合的 SpeA 的晶体结构以 3.0 Å 分辨率报道，而大肠杆菌的晶体结构SpeA 与硫酸根离子的复合物报告分辨率为 3.1 Å。SpeA 单体的结构包含两个大结构域，一个 N 端 TIM 桶结构域，然后是一个 β 夹心结构域，以及两个较小的螺旋结构域。TIM-barrel 和 β-sandwich 结构域与其他几种 PLP 依赖性脱羧酶具有结构同源性，即使这些酶之间的序列保守性低于 25%。在空肠弯曲杆菌和大肠杆菌中观察到类似的四聚体SpeA，由紧密结合的单体的两个二聚体组成。SpeA 的活性位点位于该二聚体的界面，由一个单体的 TIM 桶结构域的残基和另一个单体的 β 夹心结构域中的高度保守环形成。PLP 辅因子通过氢键、π 堆积和范德华相互作用被识别。

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Structures of bacterial biosynthetic arginine decarboxylases.

Biosynthetic arginine decarboxylase (ADC; also known as SpeA) plays an important role in the biosynthesis of polyamines from arginine in bacteria and plants. SpeA is a pyridoxal‐5′‐phosphate (PLP)‐dependent enzyme and shares weak sequence homology with several other PLP‐dependent decarboxylases. Here, the crystal structure of PLP‐bound SpeA from Campylobacter jejuni is reported at 3.0 Å resolution and that of Escherichia coli SpeA in complex with a sulfate ion is reported at 3.1 Å resolution. The structure of the SpeA monomer contains two large domains, an N‐terminal TIM‐barrel domain followed by a β‐sandwich domain, as well as two smaller helical domains. The TIM‐barrel and β‐sandwich domains share structural homology with several other PLP‐dependent decarboxylases, even though the sequence conservation among these enzymes is less than 25%. A similar tetramer is observed for both C. jejuni and E. coli SpeA, composed of two dimers of tightly associated monomers. The active site of SpeA is located at the interface of this dimer and is formed by residues from the TIM‐barrel domain of one monomer and a highly conserved loop in the β‐sandwich domain of the other monomer. The PLP cofactor is recognized by hydrogen‐bonding, π‐stacking and van der Waals interactions.

更新日期：2010-12-07

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