1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine (MPTP) is a neurotoxin that causes Parkinson's disease in animals via mechanisms linked with oxidative stress and inflammation. Resveratrol is a natural polyphenol and a phytoalexin with antioxidative and antiinflammatory properties. Here, we investigated the rescue role of resveratrol on MPTP-triggered toxicity in Drosophila melanogaster for the first time. D. melanogaster (Harwich strain, 1-to 3- days old) were first orally exposed to resveratrol (0, 7.5, 15, 30, 60 and 120 mg/kg diet) and MPTP (0, 250, 500, 1000, 2000, and 3000 μM) for longevity and 7 days survival assays respectively. Consequently, we selected resveratrol (30 and 60 mg/kg diet) to evaluate its rescue role on MPTP (250 and 500 μM)-induced toxicity in D. melanogaster after 3 days of oral treatment. Specifically, we evaluated markers of neurotoxicity (acetylcholinesterase and negative geotaxis), inflammation (nitric oxide), oxidative stress-antioxidant status (hydrogen peroxide, total thiol, catalase and glutathione-S-transferase), cell viability and fecundity. The data showed that resveratrol increased lifespan of D. melanogaster in a dose-dependent manner up to 60 mg/kg diet. Further, resveratrol restored MPTP-induced inhibition of catalase, glutathione-S-transferase and acetylcholinesterase activities in D. melanogaster. Moreover, resveratrol ameliorated MPTP-triggered cell death, histological alterations, behavioural deficits and accumulation of nitric oxide and hydrogen peroxide levels in flies (p < 0.05). Conclusively, the lifespan extension effects of resveratrol and its rescue role on MPTP- mediated toxicity in the flies may be due to its antioxidant and anti-inflammatory properties.

中文翻译：

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白藜芦醇延长了寿命并改善了果蝇中1-甲基-4-苯基-1,2,3,6-四氢吡啶诱导的氧化损伤和行为缺陷。

1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）是一种神经毒素，可通过与氧化应激和炎症相关的机制在动物中引起帕金森氏病。白藜芦醇是一种天然多酚和一种植物抗毒素，具有抗氧化和抗炎特性。在这里，我们首次调查了白藜芦醇对果蝇MPTP触发的毒性的挽救作用。首先将D. melanogaster（Harwich菌株，1-3天大）口服白藜芦醇（0，7.5，15，30，60和120 mg / kg饮食）和MPTP（0，250，500，1000，2000 ，和3000μM）分别用于寿命和7天生存期分析。因此，我们选择白藜芦醇（30和60 mg / kg饮食）以评估口服治疗3天后其对MPTP（250和500μM）诱导的D. melanogaster毒性的挽救作用。特别，我们评估了神经毒性（乙酰胆碱酯酶和负趋性性），炎症（一氧化氮），氧化应激-抗氧化剂状态（过氧化氢，总硫醇，过氧化氢酶和谷胱甘肽-S-转移酶），细胞活力和繁殖力的标记。数据显示，白藜芦醇以剂量依赖的方式增加了黑腹果蝇的寿命，直至60 mg / kg饮食。此外，白藜芦醇恢复了MPTP诱导的黑腹果蝇中过氧化氢酶，谷胱甘肽-S-转移酶和乙酰胆碱酯酶活性的抑制。此外，白藜芦醇改善了MPTP触发的细胞死亡，组织学改变，行为缺陷以及果蝇中一氧化氮和过氧化氢水平的积累（p <0.05）。最后，