Val-ala-pro-gly，一种弹性蛋白衍生的非整联蛋白配体：平滑肌细胞粘附和特异性。,Journal of Biomedical Materials Research Part A

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Val-ala-pro-gly，一种弹性蛋白衍生的非整联蛋白配体：平滑肌细胞粘附和特异性。
Journal of Biomedical Materials Research Part A ( IF 3.9 ) Pub Date : 2003-10-01 , DOI: 10.1002/jbm.a.10110
Andrea S Gobin ₁ , Jennifer L West

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弹性蛋白衍生的肽val-ala-pro-gly（VAPG）可用作平滑肌细胞的生物特异性细胞粘附配体。通过将肽序列嫁接到水凝胶材料中，我们能够评估其对平滑肌细胞粘附和扩散的影响。这些材料是基于聚乙二醇（PEG）丙烯酸酯末端衍生物的可光聚合水凝胶。由于它们的高PEG含量，这些材料对蛋白质的吸附和细胞粘附具有很高的抵抗力。但是，可以将PEG二丙烯酸酯衍生物与粘附肽修饰的PEG单丙烯酸酯衍生物混合，以促进细胞粘附。光聚合后，将PEG单丙烯酸酯衍生物接枝到由PEG二丙烯酸酯形成的水凝胶网络中。这导致粘附肽通过柔性接头链共价固定在水凝胶上。PEG对蛋白质吸附的抵抗力使其成为该模型系统的理想材料，因为细胞与材料之间的相互作用仅限于共价结合到材料中的生物分子。在这种情况下，我们能够证明VAPG对平滑肌细胞的粘附具有特异性。还显示成纤维细胞，内皮细胞和血小板不能粘附于VAPG。另外，不仅平滑肌细胞粘附依赖于配体浓度，而且细胞扩散也随着配体浓度的增加而增加。PEG对蛋白质吸附的抵抗力使其成为该模型系统的理想材料，因为细胞与材料之间的相互作用仅限于共价结合到材料中的生物分子。在这种情况下，我们能够证明VAPG对平滑肌细胞的粘附具有特异性。还显示成纤维细胞，内皮细胞和血小板不能粘附于VAPG。另外，不仅平滑肌细胞粘附依赖于配体浓度，而且细胞扩散也随着配体浓度的增加而增加。PEG对蛋白质吸附的抵抗力使其成为该模型系统的理想材料，因为细胞与材料之间的相互作用仅限于共价结合到材料中的生物分子。在这种情况下，我们能够证明VAPG对平滑肌细胞的粘附具有特异性。还显示成纤维细胞，内皮细胞和血小板不能粘附于VAPG。另外，不仅平滑肌细胞粘附依赖于配体浓度，而且细胞扩散也随着配体浓度的增加而增加。

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Val-ala-pro-gly, an elastin-derived non-integrin ligand: smooth muscle cell adhesion and specificity.

The elastin-derived peptide val-ala-pro-gly (VAPG) may be useful as a biospecific cell adhesion ligand for smooth muscle cells. By grafting the peptide sequence into a hydrogel material, we were able to assess its effects on smooth muscle cell adhesion and spreading. These materials are photopolymerizable hydrogels based on acrylate-terminated derivatives of polyethylene glycol (PEG). Because of their high PEG content, these materials are highly resistant to protein adsorption and cell adhesion. However, PEG diacrylate derivatives can be mixed with adhesive peptide-modified PEG monoacrylate derivatives to facilitate cell adhesion. Following photopolymerization, PEG monoacrylate derivatives are grafted into the hydrogel network formed by the PEG diacrylate. This results in covalent immobilization of adhesive peptides to the hydrogel via a flexible linker chain. The resistance of PEG to protein adsorption makes it an ideal material for this model system since cell-material interactions are limited to biomolecules that are covalently incorporated into the material. In this case we were able to demonstrate that VAPG is specific for adhesion of smooth muscle cells. It also was shown that fibroblasts, endothelial cells, and platelets cannot adhere to VAPG. In addition, not only was smooth muscle cell adhesion dependent on ligand concentration, but also cell spreading increased with increasing ligand concentration.

更新日期：2019-11-01

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