OBJECTIVE To describe the distribution of high-pathogenicity island (HPI) of Yersinia enterocolitica in enterotoxingenic E.coli (ETEC) and enteropathogenic E.coli (EPEC), and to understand the structure and function of HPI. METHODS PCR was used to detect irp2, fyua and asn-intB genes with subsequent sequence analysis of these genes. Nucleic acid in situ hybridization was employed to identify the specificity of irp2 and fyua. RESULTS Thirty irp2-positive strains were isolated from 93 ETEC strains and 3 from 10 EPEC strains, making a positivity rate of 32.25% and 30% respectively, and the positivity rates of fyua gene in ETEC and EPEC were 21.51% and 30% respectively. In most of these positive isolates, HPI was bordered by an asn tRNA locus, as in Yersinia sp. CONCLUSIONS This study demonstrates that the high positivity rate of HPI of Yersinia enterocolitica in ETEC and EPEC strains may be crucial to the virulence changes, virulence evolution and virulence regulation in E.coli.

中文翻译：

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在肠毒素和肠致病性大肠杆菌菌株中检​​测到小肠结肠炎耶尔森菌的高致病性岛。

目的描述小肠结肠炎耶尔森菌的高致病性岛（HPI）在肠毒素性大肠杆菌（ETEC）和肠致病性大肠杆菌（EPEC）中的分布，并了解HPI的结构和功能。方法采用PCR技术检测irp2，fyua和asn-intB基因，并对这些基因进行后续序列分析。核酸原位杂交用于鉴定irp2和fyua的特异性。结果从93个ETEC菌株中分离出30株irp2阳性菌株，从10个EPEC菌株中分离出3株，阳性率分别为32.25％和30％，fyua基因在ETEC和EPEC中的阳性率分别为21.51％和30％。在大多数阳性分离物中，HPI与耶尔森菌（Yersinia sp。）中的asn tRNA基因座相邻。