A new derivative of isochroman, 7-(isopropoxymethyl)-5-phenyl-7,8-dihydro-5H-[1,3]dioxolo[4,5-g]isochromene (ISO-9), was synthesized in our laboratory. In this study, we investigated the effect of ISO-9 on the apoptosis induced by deprivation of serum and fibroblast growth factor-2 (FGF-2) in human umbilical vein vascular endothelial cells (HUVECs). The results of MTT assay showed that 40 microM ISO-9 prevented the reduction of cell viability induced by the deprivation of serum and FGF-2 at 24 h or 48 h, respectively. To further study the correlated mechanism, the levels of integrin beta4, p53 and reactive oxygen species (ROS) were analyzed. The results showed that the high levels of integrin beta4, p53 and ROS induced by the deprivation of serum and FGF-2 could be inhibited by the treatment of 40 microM ISO-9. The data suggested that ISO-9 was a promising anti-apoptotic agent and could be served as a useful tool to study the molecular mechanism of apoptosis in vascular endothelial cells (VECs).

中文翻译：

---

一种新型的异色满衍生物通过降低整合素beta4，p53和ROS的水平来抑制血管内皮细胞的凋亡。

在我们的实验室中合成了一种新的异色满衍生物，即7-（异丙氧基甲基）-5-苯基-7,8-二氢-5H- [1,3]二氧代[4,5-g]异戊二烯（ISO-9）。在这项研究中，我们调查了ISO-9对人脐静脉血管内皮细胞（HUVECs）血清和成纤维细胞生长因子2（FGF-2）缺乏引起的凋亡的影响。MTT分析的结果表明，40 microM ISO-9分别防止了在24 h或48 h血清和FGF-2缺乏引起的细胞活力降低。为了进一步研究相关机制，分析了整联蛋白β4，p53和活性氧（ROS）的水平。结果表明，通过40 microM ISO-9处理可以抑制血清和FGF-2缺乏引起的高水平的整合素β4，p53和ROS。