Transient expression is a rapid, useful approach for producing proteins of interest in plants. Tobacco mosaic virus (TMV)-based transient expression vectors can express very high levels of foreign proteins in plants. However, TMV vectors are, in general, not efficiently delivered to plant cells by agroinfection. It was determined that agroinfection was very efficient with a 35S promoter-driven TMV replicon that lacked the TMV coat protein gene sequence. This coat protein deletion vector had several useful features as a transient expression system, including improved ease of use, higher protein expression rates, and improved biocontainment. Using this TMV expression vector, some foreign proteins were expressed at levels of 3 to 5 mg/g fresh weight of plant tissue. It is proposed that this new transient expression vector will be a useful tool for expressing recombinant proteins in plants for either research or production purposes.

中文翻译：

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TRBO：一种高效的基于烟草花叶病毒RNA的过表达载体。

瞬时表达是一种在植物中产生目标蛋白质的快速，有用的方法。基于烟草花叶病毒（TMV）的瞬时表达载体可以在植物中表达很高水平的外源蛋白质。然而，一般而言，TMV载体不能通过农业感染有效地递送至植物细胞。已确定，使用缺少TMV外壳蛋白基因序列的35S启动子驱动的TMV复制子，农业感染非常有效。这种外壳蛋白缺失载体具有作为瞬时表达系统的几种有用功能，包括提高的易用性，更高的蛋白表达率和更高的生物容纳度。使用该TMV表达载体，一些外源蛋白以植物组织新鲜重量的3至5mg / g的水平表达。