水稻WRKY45在苯并噻二唑诱导的抗病性中起着至关重要的作用。,The Plant Cell

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水稻WRKY45在苯并噻二唑诱导的抗病性中起着至关重要的作用。
The Plant Cell ( IF 10.0 ) Pub Date : 2007-07-03 , DOI: 10.1105/tpc.106.046250
Masaki Shimono ₁ , Shoji Sugano , Akira Nakayama , Chang-Jie Jiang , Kazuko Ono , Seiichi Toki , Hiroshi Takatsuji

Affiliation

苯并噻二唑（BTH）是一种所谓的植物激活剂，可通过激活水杨酸（SA）信号传导途径保护植物免受疾病侵害。通过微阵列筛选，我们鉴定出BTH和SA诱导的WRKY转录因子（TF）基因在BTH治疗后3小时内被上调。其中之一WRKY45在水稻（Oryza sativa）中的过表达显着增强了对稻瘟病真菌的抗性。RNA干扰介导的WRKY45的敲低损害了BTH诱导的对瘟病的抗性，表明它对BTH诱导的防御反应至关重要。在瞬时表达系统中，WRKY45通过W-box激活了报告基因的转录。上位性分析表明WRKY45在SA信号传导途径中的作用独立于拟南芥NPR1的水稻直系同源基因NH1，这使WRKY45与已知的拟南芥WRKY TF区别开来。发现与防御相关的两个基因，编码谷胱甘肽S-转移酶和细胞色素P450，在WRKY45下游受到调控，但不受NH1调控，这与WRKY45和NH1依赖途径的明显独立性是一致的。过量表达WRKY45的水稻植株中的防御基因表达随生长条件而变化，这表明某些环境因素在WRKY45转录的下游起作用。我们提出WRKY45在BTH诱导的和SA介导的水稻防御信号中的作用及其在提高水稻抗病性方面的潜在效用，水稻是全球重要的食品资源。被发现受WRKY45下游调控，但不受NH1调控，这与WRKY45和NH1依赖途径的明显独立性是一致的。过量表达WRKY45的水稻植株中的防御基因表达随生长条件而变化，这表明某些环境因素在WRKY45转录的下游起作用。我们提出WRKY45在BTH诱导的和SA介导的水稻防御信号中的作用及其在提高水稻抗病性方面的潜在效用，水稻是全球重要的食品资源。被发现受WRKY45下游调控，但不受NH1调控，这与WRKY45和NH1依赖途径的明显独立性是一致的。过量表达WRKY45的水稻植株中的防御基因表达随生长条件而变化，这表明某些环境因素在WRKY45转录的下游起作用。我们提出WRKY45在BTH诱导的和SA介导的水稻防御信号中的作用及其在提高水稻抗病性方面的潜在效用，水稻是全球重要的食品资源。

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Rice WRKY45 plays a crucial role in benzothiadiazole-inducible blast resistance.

Benzothiadiazole (BTH) is a so-called plant activator and protects plants from diseases by activating the salicylic acid (SA) signaling pathway. By microarray screening, we identified BTH- and SA-inducible WRKY transcription factor (TF) genes that were upregulated within 3 h after BTH treatment. Overexpression of one of them, WRKY45, in rice (Oryza sativa) markedly enhanced resistance to rice blast fungus. RNA interference-mediated knockdown of WRKY45 compromised BTH-inducible resistance to blast disease, indicating that it is essential for BTH-induced defense responses. In a transient expression system, WRKY45 activated reporter gene transcription through W-boxes. Epistasis analysis suggested that WRKY45 acts in the SA signaling pathway independently of NH1, a rice ortholog of Arabidopsis thaliana NPR1, which distinguishes WRKY45 from known Arabidopsis WRKY TFs. Two defense-related genes, encoding a glutathione S-transferase and a cytochrome P450, were found to be regulated downstream of WRKY45 but were not regulated by NH1, consistent with the apparent independence of the WRKY45- and NH1-dependent pathways. Defense gene expression in WRKY45-overexpressed rice plants varied with growth conditions, suggesting that some environmental factor(s) acts downstream of WRKY45 transcription. We propose a role for WRKY45 in BTH-induced and SA-mediated defense signaling in rice and its potential utility in improving disease resistance of rice, an importance food resource worldwide.

更新日期：2019-11-01

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