WC1-和WC1 +反刍动物γT细胞亚群（包括那些对细菌抗原有反应的亚种）之间的TCR基因使用差异。,Immunogenetics

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WC1-和WC1 +反刍动物γT细胞亚群（包括那些对细菌抗原有反应的亚种）之间的TCR基因使用差异。
Immunogenetics ( IF 2.9 ) Pub Date : 2006-06-27 , DOI: 10.1007/s00251-006-0122-5
Seth L Blumerman ₁ , Carolyn T A Herzig , Aric N Rogers , Janice C Telfer , Cynthia L Baldwin

Affiliation

根据是否存在WC1共同受体（富含γ-半胱氨酸T细胞的清道夫-受体-半胱氨酸家族成员），将反刍型伽马T细胞分为亚群。有证据表明WC1 +具有炎症性，而WC1-具有调节性，并且它们的组织分布也不同。最近，随着产生干扰素-γ并增殖为自体抗原，钩端螺旋体抗原或IL-12的细胞被大量发现在带有WC1.1抗原表位但未携带WC1.2的WC1 +亚群中，这一范例得到了进一步的完善。表位。在这里，使用流式细胞仪纯化的细胞和逆转录酶-聚合酶链反应（RT-PCR）比较了这些不同亚群（WC1-，WC1.1 +和WC1.2 +）的T细胞受体基因表达。WC1-γδT细胞具有TRG亚组V和C基因的所有11种可能组合的转录本，而WC1 +亚群中的转录本均限于TRGV3-TRGC5和TRGV7-TRGC5。相反，所有三个亚群都表达了来自所有四个已知的牛TRDV基因的转录本。对钩端螺旋体抗原刺激培养物中增殖的WC1 +γT细胞的进一步分析表明，基于其TCR基因的使用，它们在较大的WC1 +群体中并不代表独特的亚群。此外，对65个转录物的测序表明，它们的连接区域各不相同，因为使用了TRGJ5-1，TRGJ5-2，TRDJ1和TRDJ3，CDR3的范围为9至24个氨基酸。WC1.1 +，WC1.2 +，

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Differential TCR gene usage between WC1- and WC1+ ruminant gammadelta T cell subpopulations including those responding to bacterial antigen.

Ruminant gammadelta T cells are divided into subpopulations based on the presence or absence of WC1 co-receptors (scavenger-receptor-cysteine-rich family members uniquely expressed on gammadelta T cells). Evidence suggests WC1+ are inflammatory while WC1- are regulatory and that they also differ in their tissue distribution. Recently, this paradigm was refined further as cells that produce interferon-gamma and proliferate to autologous antigens, leptospira antigens, or IL-12 were largely found within the WC1+ subpopulation that bears the WC1.1 antigenic epitope but not that bearing the WC1.2 epitope. Here, the T cell receptor gene expression by these different subpopulations (WC1-, WC1.1+, and WC1.2+) was compared using flow cytometrically-purified cells and reverse transcriptase-polymerase chain reaction (RT-PCR). The WC1- gammadelta T cells had transcripts for all 11 possible combinations of the TRG subgroup V and C genes while those in both WC1+ subpopulations were restricted to TRGV3-TRGC5 and TRGV7-TRGC5. In contrast, all three subpopulations expressed transcripts from all four known bovine TRDV genes. Further analysis of the WC1+ gammadelta T cells that proliferated in leptospira antigen-stimulated cultures indicated that they do not represent a unique subpopulation within the larger WC1+ population based on their TCR gene usage. Moreover, sequencing of 65 transcripts showed that their junctional regions were diverse as TRGJ5-1, TRGJ5-2, TRDJ1, and TRDJ3 were used, and CDR3s ranged from 9 to 24 amino acids. The restricted but shared gammadelta TCR gene usage for WC1.1+, WC1.2+, and WC1(+)-antigen-responsive cells leaves open the possibility that the WC1 co-receptor is an important determining element in the activation process and subsequent response.

更新日期：2019-11-01

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