<br>烟草花叶病毒 (TMV) 和马铃薯 X 病毒 (PVX) 外壳蛋白分别对 PVX 和 TMV 感染产生异源干扰。,Journal of General Virology

当前位置： X-MOL 学术 › J. Gen. Virol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

烟草花叶病毒 (TMV) 和马铃薯 X 病毒 (PVX) 外壳蛋白分别对 PVX 和 TMV 感染产生异源干扰。
Journal of General Virology ( IF 3.6 ) Pub Date : 2006-04-01 , DOI: 10.1099/vir.0.81396-0
A A Bazzini _{1,

2} , S Asurmendi _{1,

2} , H E Hopp ₁ , R N Beachy ₂

Affiliation

马铃薯病毒 X (PVX) 在转基因原生质体中的复制减少，这些原生质体积累了烟草花叶病毒 (TMV) 的野生型外壳蛋白 (CPWT) 或突变体 CP、CP(T42W)，产生高度有序的聚集状态，包括假病毒粒子。该反应称为异源 CP 介导的抗性。然而，表达消除聚集且不产生假病毒体的CP突变体CPT28W的原生质体并没有减少PVX复制。类似地，在产生TMV CPWT或CP(T42W)的转基因烟草植物中，PVX感染的局部细胞间传播存在延迟，而在CP(T28W)植物或非转基因植物中未观察到这一点。结果表明，TMV CP 的四级结构调节异源 CP 介导的抗性机制。同样，产生 PVX CP 的转基因原生质体可提供针对 TMV RNA 感染的短暂保护。积累PVX CP的转基因植物减少了感染在细胞间的传播，并导致接种TMV或TMV RNA后全身感染的延迟。异源CP介导的耐药性的特征是全身感染的短暂延迟，而同源CP介导的耐药性则导致全身感染减少或无全身感染。

"点击查看英文标题和摘要"

Tobacco mosaic virus (TMV) and potato virus X (PVX) coat proteins confer heterologous interference to PVX and TMV infection, respectively.

Replication of Potato virus X (PVX) was reduced in transgenic protoplasts that accumulated wild-type coat protein (CPWT) of Tobacco mosaic virus (TMV) or a mutant CP, CP(T42W), that produced highly ordered states of aggregation, including pseudovirions. This reaction is referred to as heterologous CP-mediated resistance. However, protoplasts expressing a CP mutant that abolished aggregation and did not produce pseudovirions, CPT28W, did not reduce PVX replication. Similarly, in transgenic tobacco plants producing TMV CPWT or CP(T42W), there was a delay in local cell-to-cell spread of PVX infection that was not observed in CP(T28W) plants or in non-transgenic plants. The results suggest that the quaternary structure of the TMV CP regulates the mechanism(s) of heterologous CP-mediated resistance. Similarly, transgenic protoplasts that produced PVX CP conferred transient protection against infection by TMV RNA. Transgenic plants that accumulated PVX CP reduced the cell-to-cell spread of infection and resulted in a delay in systemic infection following inoculation with TMV or TMV RNA. Heterologous CP-mediated resistance was characterized by a brief delay in systemic infection, whilst homologous CP-mediated resistance conferred reduced or no systemic infection.

更新日期：2019-11-01

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南