组胺受体 H1R、H2R 和 H4R（而非 H3R）在人肠道中的选择性表达,Gut

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组胺受体 H1R、H2R 和 H4R（而非 H3R）在人肠道中的选择性表达
Gut ( IF 23.0 ) Pub Date : 2006-04-01 , DOI: 10.1136/gut.2004.061762
L E Sander ₁ , A Lorentz , G Sellge , M Coëffier , M Neipp , T Veres , T Frieling , P N Meier , M P Manns , S C Bischoff

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背景和目的：组胺被认为是胃肠道功能的调节剂，如胃酸产生、肠蠕动和粘膜离子分泌。这些知识大部分是从动物研究中获得的。相比之下，在人类中，人类胃肠道内组胺受体 (HR) 的表达和分布尚不清楚。方法：我们通过定量逆转录聚合酶链反应和免疫染色分析了人类胃肠道组织标本中的 HR 表达。结果：我们发现 H1R、H2R 和 H4R mRNA 在整个胃肠道中均有表达，而 H3R mRNA 不存在。不同解剖部位（十二指肠、回肠、结肠、sigma 和直肠）的 HR 分布没有显着差异。神经元和神经纤维的免疫染色显示人类肠道神经系统中不存在 H3R；然而，在肌间神经丛的神经节细胞上发现了 H1R 和 H2R。上皮细胞也表达 H1R、H2R，在某种程度上，还表达 H4R。肠道成纤维细胞只表达 H1R，而人类肠道的肌肉层对 H1R 和 H2R 染色呈阳性。免疫细胞表达 H1R、H2R 和低水平 H4R 的 mRNA 和蛋白质。对食物过敏和肠易激综合征患者的内窥镜活检分析显示，与对照组相比，H1R 和 H2R mRNA 水平显着升高。结论：我们已经证明 H1R、H2R 和在某种程度上 H4R 在人类胃肠道中表达，而 H3R 不存在，

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Selective expression of histamine receptors H1R, H2R, and H4R, but not H3R, in the human intestinal tract

Background and aims: Histamine is known as a regulator of gastrointestinal functions, such as gastric acid production, intestinal motility, and mucosal ion secretion. Most of this knowledge has been obtained from animal studies. In contrast, in humans, expression and distribution of histamine receptors (HR) within the human gastrointestinal tract are unclear. Methods: We analysed HR expression in human gastrointestinal tissue specimens by quantitative reverse transcription-polymerase chain reaction and immunostaining. Results: We found that H1R, H2R, and H4R mRNA were expressed throughout the gastrointestinal tract, while H3R mRNA was absent. No significant differences in the distribution of HR were found between different anatomical sites (duodenum, ileum, colon, sigma, and rectum). Immunostaining of neurones and nerve fibres revealed that H3R was absent in the human enteric nervous system; however, H1R and H2R were found on ganglion cells of the myenteric plexus. Epithelial cells also expressed H1R, H2R and, to some extent, H4R. Intestinal fibroblasts exclusively expressed H1R while the muscular layers of human intestine stained positive for both H1R and H2R. Immune cells expressed mRNA and protein for H1R, H2R, and low levels of H4R. Analysis of endoscopic biopsies from patients with food allergy and irritable bowel syndrome revealed significantly elevated H1R and H2R mRNA levels compared with controls. Conclusions: We have demonstrated that H1R, H2R and, to some extent, H4R, are expressed in the human gastrointestinal tract, while H3R is absent, and we found that HR expression was altered in patients with gastrointestinal diseases.

更新日期：2006-04-01

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