UVR-B诱导C57小鼠白内障发展。,Experimental Eye Research

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UVR-B诱导C57小鼠白内障发展。
Experimental Eye Research ( IF 3.0 ) Pub Date : 2005-09-28 , DOI: 10.1016/j.exer.2005.02.009
Linda M Meyer ₁ , Per Söderberg , Xiuqin Dong , Alfred Wegener

Affiliation

研究了暴露于B型紫外线（UVR-B）后C57小鼠晶状体的形态外观和光散射强度的变化。将总共80只6周大的雌性C57BL / 6小鼠分为四组（n = 20）。将每只动物的一只眼睛在体内暴露于300 nm波长区域（UVR-B-300 nm）中的UVR-B，剂量为5 kJm（-2）15分钟。辐射输出在302 nm和5 nm [FWHM]下具有lambda（max）。在暴露后1、2、4和8天连续处死动物。使用网格和暗视野照明摄影记录了宏观镜头的变化。定量测量暴露的和对侧未暴露的镜片中的光散射。使用网格和暗场照明摄影记录了晶状体的变化。在所有研究的时间点，体内暴露于所有裸露晶状体的UVR-B-300 nm诱导的囊下白内障，偶尔出现皮质和核白内障。与对侧非曝光镜片相比，在所有调查日内，曝光镜片的散射光明显更高。在暴露的以及对侧未暴露的晶状体中，在第2天发现了光散射峰值的瞬时增加。镜头的光散射随曝光后的等待时间而变化。5 kJm（-2）UVR-B-300 nm的剂量在C57小鼠晶状体中引起光散射。该增加在暴露后2天有一个瞬时峰值。在第1、2、4和8天之间光散射的变化表明，在小鼠晶状体单方面体内暴露于5 kJm（-2）UVR-B-300 nm后，散射特性的动态变化。

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UVR-B induced cataract development in C57 mice.

The evolution of the morphological appearance and intensity of light scattering in C57 mice lenses after exposure to ultraviolet radiation type B (UVR-B) was investigated. A total of 80, 6-week-old female C57BL/6 mice were divided into four groups (n=20). One eye in each animal was exposed in vivo to UVR-B in the 300 nm wavelength region (UVR-B-300 nm) to a dose of 5 kJm(-2) for 15 min. The radiation output had lambda(max) at 302 nm with 5 nm [FWHM]. The animals were consecutively sacrificed at 1, 2, 4 and 8 days after the exposure. Macroscopic lens changes were documented using grid- and dark field illumination photography. Light scattering in the exposed and contralateral not exposed lens was measured quantitatively. Morphological lens changes were documented using grid- and dark field illumination photography. In vivo exposure to UVR-B-300 nm induced subcapsular cataract in all exposed lenses and occasionally cortical and nuclear cataract at all investigated time points. Exposed lenses scattered light significantly higher on all investigated days compared to contralateral non-exposed lenses. A transient increase of light scattering peaking at day 2 in exposed as well as in contralateral not exposed lenses was identified. Light scattering of the lenses varies with latency time after exposure. A dose of 5 kJm(-2) UVR-B-300 nm induces light scattering in C57 mice lenses. The increase has a transient peak at 2 days after exposure. The variation of light scattering among days 1, 2, 4, and 8 indicates a dynamic change of scattering characteristics in the mouse lens following unilateral in vivo exposure to 5 kJm(-2) UVR-B-300 nm.

更新日期：2019-11-01

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