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New fluorochromes for thiols: maleimide and iodoacetamide derivatives of a 3-phenylcoumarin fluorophore.
Journal of Histochemistry & Cytochemistry ( IF 1.9 ) Pub Date : 1981-02-01 , DOI: 10.1177/29.2.7019305
T O Sippel

N-(4-(7 - Diethylamino - methylcoumarin - 3 yl)phenyl)maleimide (CPM) and the corresponding iodoacetamide are described. When applied to sections at pH 6 and 9, respectively, the two fluorogens are very similar in their reactivity and selectivity toward thiols with which they form adducts having much the same bright blue fluorescence. However, difference in other properties favor CPM for further study.

中文翻译:

用于硫醇的新型荧光染料:3-苯基香豆素荧光团的马来酰亚胺和碘乙酰胺衍生物。

描述了N-(4-(7-二乙氨基-甲基香豆素-3基)苯基)马来酰亚胺(CPM)和相应的碘乙酰胺。当分别应用于pH 6和9的部分时,这两种氟的反应活性和对硫醇的选择性非常相似,它们与之形成的加合物具有几乎相同的亮蓝色荧光。但是,其他属性的差异有利于CPM进一步研究。
更新日期:2019-11-01
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