Treatment of Friend leukemia cells for 18 hours with 9,10-anthracenedione, 1,4-bis[[(2-hydroxyethyl)amino]ethyl]amino]-, diacetate (ANT) at concentrations up to 1.0 microgram/ml induced significant changes in cell metabolism and structure. Alterations in cell nucleic acid content were detected in cells stained with acridine orange under conditions such that DNA and RNA contents could be measured simultaneously by flow cytometry. Cells treated for 18 hours with ANT at concentrations of 0.05-0.1 microgram/ml became partially blocked at the G2 phase. In addition, about 30% of the cells became polyploid and demonstrated diplochromosomes at the 8C level of mitosis. The nuclear chromatin of blocked cells had an altered structure as reflected by a change in sensitivity of DNA in situ to denaturation induced by low pH. All viable cells treated with ANT for 18 hours at concentrations of 0.4-1.0 microgram/ml were blocked in G2 phase. These cells had significantly more RNA than did untreated cells. Transmission electron microscopic observations of thin-sectioned cells suggested that this increased RNA content in ANT-treated cells was mostly due to an approximately 50% increased cell diameter and partly due to a disproportionate increase in nucleolar size. In addition, electron microscopy revealed that ANT caused increased chromatin condensation and granulation. The drug had no apparent effect on production of the endogenous Friend murine leukemia virus.

中文翻译：

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9,10-蒽二酮，1,4-双[[2-[（[2-[（2-羟乙基）氨基]-乙基]氨基]-二乙酸酯对白血病细胞的形态和核酸的影响。

用浓度高达1.0微克/毫升的9,10-蒽二酮，1,4-双[[[（（（2-羟乙基）氨基]乙基]氨基]-双乙酸盐（ANT）处理Friend白血病细胞18小时，诱导了显着变化在细胞代谢和结构。在可通过流式细胞术同时测量DNA和RNA含量的条件下，在用a啶橙染色的细胞中检测到细胞核酸含量的变化。用ANT以0.05-0.1微克/毫升的浓度处理18小时的细胞在G2期被部分封闭。此外，约有30％的细胞变为多倍体，并在8C有丝分裂水平显示出双色染色体。封闭细胞的核染色质具有改变的结构，这反映在原位DNA对低pH诱导的变性的敏感性变化中。用ANT以0.4-1.0微克/毫升的浓度处理18小时的所有活细胞均被阻滞在G2期。这些细胞比未处理的细胞具有更多的RNA。透射电子显微镜对薄层细胞的观察表明，这种在ANT处理过的细胞中增加的RNA含量主要是由于细胞直径增加了约50％，部分原因是核仁大小不成比例地增加。此外，电子显微镜显示，ANT导致染色质浓缩和颗粒化增加。该药物对内源性Friend鼠白血病病毒的产生没有明显作用。透射电子显微镜对薄层细胞的观察表明，这种在ANT处理过的细胞中增加的RNA含量主要是由于细胞直径增加了约50％，部分原因是核仁大小不成比例地增加。此外，电子显微镜显示，ANT导致染色质浓缩和颗粒化增加。该药物对内源性Friend鼠白血病病毒的产生没有明显作用。透射电子显微镜对薄层细胞的观察表明，这种在ANT处理过的细胞中增加的RNA含量主要是由于细胞直径增加了约50％，部分原因是核仁大小不成比例地增加。此外，电子显微镜显示，ANT导致染色质浓缩和颗粒化增加。该药物对内源性Friend鼠白血病病毒的产生没有明显作用。