从转铁蛋白释放铁的动力学在内体 pH 下与转铁蛋白受体结合。,Biochimica et Biophysica Acta (BBA) - General Subjects

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从转铁蛋白释放铁的动力学在内体 pH 下与转铁蛋白受体结合。
Biochimica et Biophysica Acta (BBA) - General Subjects ( IF 2.8 ) Pub Date : 2011-06-15 , DOI: 10.1016/j.bbagen.2011.06.003
Ashley N Steere ₁ , Shaina L Byrne , N Dennis Chasteen , Anne B Mason

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背景人血清转铁蛋白(hTF) 是一种双叶糖蛋白，其可逆地结合Fe(3+) 并通过受体介导的内吞作用将其递送至细胞。尽管进行了数十年的研究，但尚未完全描述导致内体内每个 hTF 叶释放铁的确切事件。综述范围我们概述了在内体 pH 值 (5.6) 下从 hTF±转铁蛋白受体 (TFR) 释放铁的动力学。提供了用于确定准确速率常数的一系列生物物理技术的关键评估。一般意义通过 hTF 将 Fe(3+) 输送到活跃分裂的细胞是必不可少的。Fe(3+) 过多或过少都会直接影响个人的幸福感。因为 hTF 与 TFR 的相互作用控制着体内铁的分布，在分子水平上了解这一过程至关重要。主要结论 TFR 不仅通过 hTF 的结合指导铁向细胞的输送，动力学数据表明它还调节铁从 hTF 的 N 叶和 C 叶释放。具体来说，TFR 平衡了每个叶的铁释放率，从而在生理相关的时间范围内有效地释放 Fe(3+)。本文是题为“铁运输和障碍的分子机制”的特刊的一部分。导致在生理相关时间范围内有效释放 Fe(3+)。本文是题为“铁运输和障碍的分子机制”的特刊的一部分。导致在生理相关时间范围内有效释放 Fe(3+)。本文是题为“铁运输和障碍的分子机制”的特刊的一部分。

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Kinetics of iron release from transferrin bound to the transferrin receptor at endosomal pH.

BACKGROUND Human serum transferrin (hTF) is a bilobal glycoprotein that reversibly binds Fe(3+) and delivers it to cells by the process of receptor-mediated endocytosis. Despite decades of research, the precise events resulting in iron release from each lobe of hTF within the endosome have not been fully delineated. SCOPE OF REVIEW We provide an overview of the kinetics of iron release from hTF±the transferrin receptor (TFR) at endosomal pH (5.6). A critical evaluation of the array of biophysical techniques used to determine accurate rate constants is provided. GENERAL SIGNIFICANCE Delivery of Fe(3+)to actively dividing cells by hTF is essential; too much or too little Fe(3+) directly impacts the well-being of an individual. Because the interaction of hTF with the TFR controls iron distribution in the body, an understanding of this process at the molecular level is essential. MAJOR CONCLUSIONS Not only does TFR direct the delivery of iron to the cell through the binding of hTF, kinetic data demonstrate that it also modulates iron release from the N- and C-lobes of hTF. Specifically, the TFR balances the rate of iron release from each lobe, resulting in efficient Fe(3+) release within a physiologically relevant time frame. This article is part of a Special Issue entitled Molecular Mechanisms of Iron Transport and Disorders.

更新日期：2019-11-01

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