控制枯草芽孢杆菌生物膜形成的组氨酸激酶的空间调节。,Journal of Bacteriology

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控制枯草芽孢杆菌生物膜形成的组氨酸激酶的空间调节。
Journal of Bacteriology ( IF 2.7 ) Pub Date : 2010-11-19 , DOI: 10.1128/jb.01186-10
Anna L McLoon ₁ , Ilana Kolodkin-Gal , Shmuel M Rubinstein , Roberto Kolter , Richard Losick

Affiliation

由于细胞外基质的产生，枯草芽孢杆菌能够在固体培养基上形成结构复杂的生物膜。控制参与基质合成的基因表达的主要调节因子是 Spo0A，它通过涉及多个组氨酸激酶的磷酸化作用被磷酸化激活。在这里我们报告了四种激酶，KinA、KinB、KinC 和 KinD，有助于控制生物膜的形成，但它们的贡献部分被冗余掩盖了。我们表明激酶分为两类，每一对的成员（一对包含 KinA 和 KinB，另一对包含 KinC 和 KinD）彼此部分冗余。我们还表明激酶在空间上受到调节：KinA 和 KinB 主要在菌落的较旧的内部区域活跃，KinC 和 KinD 主要在较年轻的外围区域起作用。这些结论基于激酶突变体的形态、使用荧光素酶作为报告基因的实时基因表达测量以及使用荧光蛋白作为报告基因的共聚焦显微镜。我们的研究结果表明，来自菌落较老和较年轻区域的多个信号被激酶整合，以确定生物膜群落的整体结构。

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Spatial regulation of histidine kinases governing biofilm formation in Bacillus subtilis.

Bacillus subtilis is able to form architecturally complex biofilms on solid medium due to the production of an extracellular matrix. A master regulator that controls the expression of the genes involved in matrix synthesis is Spo0A, which is activated by phosphorylation via a phosphorelay involving multiple histidine kinases. Here we report that four kinases, KinA, KinB, KinC, and KinD, help govern biofilm formation but that their contributions are partially masked by redundancy. We show that the kinases fall into two categories and that the members of each pair (one pair comprising KinA and KinB and the other comprising KinC and KinD) are partially redundant with each other. We also show that the kinases are spatially regulated: KinA and KinB are active principally in the older, inner regions of the colony, and KinC and KinD function chiefly in the younger, outer regions. These conclusions are based on the morphology of kinase mutants, real-time measurements of gene expression using luciferase as a reporter, and confocal microscopy using a fluorescent protein as a reporter. Our findings suggest that multiple signals from the older and younger regions of the colony are integrated by the kinases to determine the overall architecture of the biofilm community.

更新日期：2019-11-01

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