A sorbent material based on a newly synthesized hydrazone ligand, 4-hydroxy-N'-[(E)-(2-hydroxyphenyl)methylidene]benzohydrazide was prepared by immobilizing the ligand into a silica sol-gel matrix. The capability of the sorbent material for the extraction of seven biogenic amines (BAs), i.e., tryptamine (TRY), beta-phenylethylamine (PEA), putrescine (PUT), cadaverine (CAD), histamine (HIS), tyramine (TYR), and spermidine (SPD) was studied. Under the adopted conditions, the sorbent showed good selectivity towards PUT, CAD, HIS and SPD (% extraction (%E)>96) while %E for TYR, TRY and PEA were 82.0, 78.9 and 46.4%, respectively. The sorbent could be used up to six extraction cycles for SPD, CAD and PUT and was applied to the determination of food samples ("budu", ketchup, orange juice, soy sauce) that were spiked with 20 mg L(-1) of the BAs. The extracted analytes were derivatized with dansyl chloride before the HPLC determination. With the exception of HIS and TYR in "budu" sample, reasonable recoveries were found for the other analytes in all the tested food samples.

中文翻译：

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一种基于4-羟基-N'-[（E）-（2-羟基苯基）亚甲基]苯并肼的吸附材料，用于萃取-HPLC测定食品中的生物胺。

通过将配体固定在二氧化硅溶胶-凝胶基质中，制备了基于新合成的ligand配体4-羟基-N'-[（E）-（2-羟基苯基）亚甲基]苯并酰肼的吸附剂材料。吸附剂材料提取七种生物胺（BAs）的能力，即色胺（TRY），β-苯乙胺（PEA），腐胺（PUT），尸胺（CAD），组胺（HIS），酪胺（TYR） ，并研究了亚精胺（SPD）。在采用的条件下，吸附剂对PUT，CAD，HIS和SPD表现出良好的选择性（％萃取（％E）> 96），而TYR，TRY和PEA的％E分别为82.0％，78.9％和46.4％。该吸附剂最多可用于SPD，CAD和PUT的六个萃取循环，并用于食品样品（“ budu”，番茄酱，橙汁，酱油）中掺入20 mg L（-1）的BA。在HPLC测定之前，将提取的分析物用丹磺酰氯衍生化。除“ budu”样品中的HIS和TYR外，所有测试食品样品中的其他分析物均具有合理的回收率。