从小鼠盲肠样本中分离细菌并描述 Bacteroides sartorii sp。十一月,Archives of Microbiology

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从小鼠盲肠样本中分离细菌并描述 Bacteroides sartorii sp。十一月
Archives of Microbiology ( IF 2.3 ) Pub Date : 2010-04-06 , DOI: 10.1007/s00203-010-0568-6
Thomas Clavel ₁ , Anja Saalfrank , Cédric Charrier , Dirk Haller

Affiliation

来自野生型和 TNFdeltaARE 小鼠的盲肠样本在含有胆盐、氨基酸或酪蛋白大肽的选择性培养基上培养。通过16S rRNA基因测序分离并鉴定了22株菌株。21 个菌株与已知细菌（Blautia spp.、无害梭菌、肠球菌、大肠杆菌、鼠乳杆菌、Parabacteroides goldsteinii 和痢疾志贺氏菌）的相似性超过 98%。另一种分离株，菌株 A-C2-0，是一种新细菌。近亲是 Bacteroides massiliensis、Bacteroides dorei 和 Bacteroides vulgatus（相似度≤94%）。菌株 A-C2-0 是一种革兰氏阴性杆菌，不形成孢子，DNA 的 G+C 含量为 41.5%。它的主要细胞脂肪酸是 C15:0 ANTEISO，它的主要呼吸醌是 MK-9。细胞是耐氧的，但只能在严格的缺氧条件下生长。它们对头孢噻肟和妥布霉素耐药。与相关拟杆菌属相比，新细菌α-阿拉伯糖苷酶阳性，谷氨酰谷氨酸芳基酰胺酶阴性，不代谢半乳糖、葡萄糖、果糖、甘露糖、棉子糖和蔗糖。因此，菌株 A-C2-0 作为拟杆菌属内一个新物种的成员值得承认，为此拟定名为 Bacteroides sartorii。类型菌株是 A-C2-0T（= DSM 21941T = CCUG 57211T）。因此，菌株 A-C2-0 作为拟杆菌属内一个新物种的成员值得承认，为此拟定名为 Bacteroides sartorii。类型菌株是 A-C2-0T（= DSM 21941T = CCUG 57211T）。因此，菌株 A-C2-0 作为拟杆菌属内一个新物种的成员值得承认，为此拟定名为 Bacteroides sartorii。类型菌株是 A-C2-0T（= DSM 21941T = CCUG 57211T）。

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Isolation of bacteria from mouse caecal samples and description of Bacteroides sartorii sp. nov

Caecal samples from wild-type and TNFdeltaARE mice were cultured on selective media containing bile salts, amino acids or casein macro-peptides. Twenty-two strains were isolated and identified by 16S rRNA gene sequencing. Twenty-one strains showed >98% similarity to known bacteria (Blautia spp., Clostridium innocuum, Enterococcus spp., Escherichia coli, Lactobacillus murinus, Parabacteroides goldsteinii and Shigella dysenteriae). One additional isolate, strain A-C2-0, was a new bacterium. The closest relatives were Bacteroides massiliensis, Bacteroides dorei and Bacteroides vulgatus (≤94% similarity). Strain A-C2-0 is a Gram-negative rod that does not form spores and has a G + C content of DNA of 41.5%. Its major cellular fatty acid is C15:0 ANTEISO, and its major respiratory quinone is MK-9. Cells are aerotolerant but grow only under strict anoxic conditions. They are resistant to cefotaxime and tobramycin. When compared with related Bacteroides spp., the new bacterium was positive for α-arabinosidase, negative for glutamyl glutamic acid arylamidase and did not metabolise galactose, glucose, fructose, mannose, raffinose and sucrose. Strain A-C2-0 therefore merits recognition as a member of a novel species within the genus Bacteroides, for which the name Bacteroides sartorii is proposed. The type strain is A-C2-0T (= DSM 21941T = CCUG 57211T).

更新日期：2010-04-06

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