OBJECTIVE To obtain carbendazim-degrading microbial strains, and to use them for bioremediation of contaminated soil. METHODS A carbendazim-degrading bacterium T2-2 was isolated from the screening of drug-tolerated mutants Trichoderma strains. High-pressure liquid chromatography-mass spectrometry (HPLC-MS) analysis showed the presence of the metabolites after shake incubation of the Trichoderma T2-2 at temperature 25 degrees C, 200 r/min in mineral salt medium that contained 100 mg/ L carbendazim. We prepared T2-2 bioremediation agents from crop straw through solid fermentation. By inoculating T2-2 in soil, we performed a bioremediation test of sterilized soil and original soil at 0.1 mg/g dry soil of carbendazim concentration and 10(7) cfu/g dry soil of inoculating amount. In addition, we also conducted a control effect experiment of T2-2 against fusarium wilt of cucumber. RESULTS The metabolites detected by HPLC-MS were 2-aminobenzimidazole, benzimidazole, and 2-aminobenxinitrile in the culture filtrate after 2 days of incubation. Carbendazim and metabolites could no longer be detected through the High-pressure liquid chromatography (HPLC) analysis in the culture filtrate after 5 days of incubation. In the soil bioremediation test, carbendazim in the sterilized soil was degraded completely after 6 days of inoculation, whereas the process only needed 4 days in original soil. It showed crop straw could function as co-metabolic substrate and promote co-metabolism of T2-2 and indigenous microorganisms. Moreover, the efficiency of T2-2 against cucumber fusarium wilt might reach 81.7%, which is superior to chemical pesticide. CONCLUSION T2-2 could degrade carbendazim in soil and thus control plant disease.

中文翻译：

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[降解多菌灵的木霉菌的表征。T2-2及其在生物修复中的应用]。

目的获得降解多菌灵的微生物菌株，并将其用于污染土壤的生物修复。方法从耐药性木霉菌株的筛选中分离出多菌灵降解菌T2-2。高压液相色谱-质谱（HPLC-MS）分析表明，木霉菌T2-2在25°C，200 r / min的含100 mg / L多菌灵的矿物盐培养基中摇动孵育后，存在代谢产物。我们通过固体发酵从农作物秸秆中制备了T2-2生物修复剂。通过在土壤中接种T2-2，我们在多菌灵浓度为0.1 mg / g干土壤和接种量为10（7）cfu / g干土壤的情况下，对灭菌土壤和原始土壤进行了生物修复测试。此外，我们还进行了T2-2对黄瓜枯萎病的防治效果试验。结果孵育2天后，滤液中的HPLC-MS检测代谢物为2-氨基苯并咪唑，苯并咪唑和2-氨基苯并腈。孵育5天后，无法通过高压液相色谱（HPLC）分析培养滤液中的多菌灵和代谢物。在土壤生物修复测试中，接种6天后，灭菌土壤中的多菌灵被完全降解，而原始土壤中该过程仅需4天。结果表明，农作物秸秆可以作为代谢的底物，促进T2-2与本地微生物的代谢。而且，T2-2对黄瓜枯萎病的防治效果可能达到81.7％，优于化学农药。