活性氧（ROS）是表皮生长因子（EGF）刺激的角膜上皮细胞增殖，粘附，迁移和伤口愈合的重要介质。,Experimental Eye Research

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活性氧（ROS）是表皮生长因子（EGF）刺激的角膜上皮细胞增殖，粘附，迁移和伤口愈合的重要介质。
Experimental Eye Research ( IF 3.0 ) Pub Date : 2009-07-29 , DOI: 10.1016/j.exer.2009.07.012
Yanan Huo ₁ , Wen-Ya Qiu , Qing Pan , Yu-Feng Yao , Kuiyi Xing , Marjorie F Lou

Affiliation

EGF是上皮细胞增殖和角膜伤口愈合所需的必需生长因子，但其分子机制尚不清楚。尽管研究表明，某些非吞噬细胞中的EGF会诱导ROS的产生，但对于ROS在角膜上皮细胞中的作用知之甚少。因此，我们使用兔或人角膜上皮细胞以及猪整个角膜器官培养物作为模型，研究了ROS在角膜细胞增殖，粘附和伤口愈合中的潜在生理作用。血清饥饿的RCE或HCE细胞中的EGF（5 ng / ml）诱导的ROS被捕获为DCFH荧光并通过共聚焦显微镜检测。当用抗氧化剂N-乙酰半胱氨酸（NAC）或甘露醇，或NADPH氧化酶（DPI）或脂氧合酶（NDGA）抑制剂预处理细胞时，ROS的升高被消除。EGF诱导的ROS生成与细胞生长以及Akt和MAPK信号通路的激活相关，而NAC消除了所有这些作用。NAC的存在极大地抑制了EGF刺激的细胞粘附或细胞培养迁移，而NAC也阻止了EGF促进的角膜器官培养上皮细胞伤口愈合。这是角膜伤口愈合中新型ROS生理功能的首次证明。

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Reactive oxygen species (ROS) are essential mediators in epidermal growth factor (EGF)-stimulated corneal epithelial cell proliferation, adhesion, migration, and wound healing.

EGF is an essential growth factor needed for epithelial cell proliferation and wound healing of the cornea, but the molecular mechanism is not understood. Although studies have shown that EGF in some non-phagocytic cells induces ROS generation, little is known about the role of ROS in corneal epithelial cells. Therefore, we examined the potential physiological role of ROS in corneal cell proliferation, adhesion and wound healing using rabbit or human corneal epithelial cells, and pig whole cornea organ culture as models. EGF (5 ng/ml)-induced ROS in serum-starved RCE or HCE cells were captured as DCFH fluorescence and detected by confocal microscopy. The elevation of ROS was eradicated when the cells were pretreated with an antioxidant N-acetylcysteine (NAC) or mannitol, or with inhibitor to NADPH oxidase (DPI), or to lipoxygenase (NDGA). EGF-induced ROS generation correlated with cell growth and activation of Akt and MAPK signaling pathways, while NAC eliminated all these effects. EGF-stimulated cell adhesion or migration in cell culture was greatly suppressed in the presence of NAC while EGF-facilitated epithelial cell wound healing in corneal organ culture was also blocked by NAC. This is the first demonstration of a novel ROS physiological function in corneal wound healing.

更新日期：2009-07-25

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