TTC、氟玉 B 和 NeuN 染色证实了大脑中动脉闭塞诱导的梗塞的演变阶段。,Journal of Neuroscience Methods

当前位置： X-MOL 学术 › J. Neurosci. Methods › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

TTC、氟玉 B 和 NeuN 染色证实了大脑中动脉闭塞诱导的梗塞的演变阶段。
Journal of Neuroscience Methods ( IF 2.7 ) Pub Date : 2009-01-09 , DOI: 10.1016/j.jneumeth.2008.12.028
Fudong Liu ₁ , Dorothy P Schafer , Louise D McCullough

Affiliation

关于如何最好地测量梗塞损伤以及大脑中动脉闭塞 (MCAO) 梗塞在组织学上完整的时间点，文献中存在相当多的争论。由于许多研究人员在神经保护研究中关注更多的慢性终点，因此在以后的时间点评估组织学损伤非常重要，以确保组织损伤测量的标准方法是准确的。为了比较急性和亚急性时间点的组织活力，我们使用 2,3,5-氯化三苯基四唑 (TTC)、氟玉 B 和 NeuN 染色来检查由 90 分钟 MCAO 诱导的梗塞的演变阶段在老鼠身上。在 MCAO 后 1.5 小时、6 小时、12 小时、24 小时、3 天和 7 天检查中风结果。从 1.5 小时到 24 小时，大脑皮层的梗死体积随时间增加，然后在中风后 24 小时到 7 天出现平台期。纹状体梗塞在 12 小时内完成。Fluoro-Jade B 染色在 24 小时达到峰值，并在 7 天时降至最低。我们的结果表明，在小鼠 MCAO 再灌注模型中，由 TTC 和 Fluoro-Jade B 测量的组织学损伤在中风后 24 小时达到峰值。TTC 染色可在中风后 7 天准确进行。神经功能缺损与结构性病变无关，而是与功能的暂时性损害相关。由于梗塞在纹状体 24 小时甚至更早完成，如果在此之后给予，即使是最有效的神经保护疗法也不太可能显示出任何功效。我们的结果表明，在小鼠 MCAO 再灌注模型中，由 TTC 和 Fluoro-Jade B 测量的组织学损伤在中风后 24 小时达到峰值。TTC 染色可在中风后 7 天准确进行。神经功能缺损与结构性病变无关，而是与功能的暂时性损害相关。由于梗塞在纹状体 24 小时甚至更早完成，如果在此之后给予，即使是最有效的神经保护疗法也不太可能显示出任何功效。我们的结果表明，在小鼠 MCAO 再灌注模型中，由 TTC 和 Fluoro-Jade B 测量的组织学损伤在中风后 24 小时达到峰值。TTC 染色可在中风后 7 天准确进行。神经功能缺损与结构性病变无关，而是与功能的暂时性损害相关。由于梗塞在纹状体 24 小时甚至更早完成，如果在此之后给予，即使是最有效的神经保护疗法也不太可能显示出任何功效。

"点击查看英文标题和摘要"

TTC, fluoro-Jade B and NeuN staining confirm evolving phases of infarction induced by middle cerebral artery occlusion.

Considerable debate exists in the literature on how best to measure infarct damage and at what point after middle cerebral artery occlusion (MCAO) infarct is histologically complete. As many researchers are focusing on more chronic endpoints in neuroprotection studies it is important to evaluate histological damage at later time points to ensure that standard methods of tissue injury measurement are accurate. To compare tissue viability at both acute and sub-acute time points, we used 2,3,5-triphenyltetrazolium chloride (TTC), Fluoro-Jade B, and NeuN staining to examine the evolving phases of infarction induced by a 90-min MCAO in mice. Stroke outcomes were examined at 1.5h, 6h, 12h, 24h, 3d, and 7d after MCAO. There was a time-dependent increase in infarct volume from 1.5h to 24h in the cortex, followed by a plateau from 24h to 7d after stroke. Striatal infarcts were complete by 12h. Fluoro-Jade B staining peaked at 24h and was minimal by 7d. Our results indicated that histological damage as measured by TTC and Fluoro-Jade B reaches its peak by 24h after stroke in a reperfusion model of MCAO in mice. TTC staining can be accurately performed as late as 7d after stroke. Neurological deficits do not correlate with the structural lesion but rather transient impairment of function. As the infarct is complete by 24h and even earlier in the striatum, even the most efficacious neuroprotective therapies are unlikely to show any efficacy if given after this point.

更新日期：2019-11-01

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南