辅助抑制因子 LUH 和衔接蛋白 SLK1 和 SLK2 参与拟南芥非生物胁迫响应基因的调控。,BMC Plant Biology

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辅助抑制因子 LUH 和衔接蛋白 SLK1 和 SLK2 参与拟南芥非生物胁迫响应基因的调控。
BMC Plant Biology ( IF 4.3 ) Pub Date : 2014-02-26 , DOI: 10.1186/1471-2229-14-54
Barsha Shrestha , Bhuwan Guragain , Vaniyambadi V Sridhar ₁

Affiliation

背景在非生物胁迫期间，许多对生长和适应胁迫很重要的基因以升高的水平表达。然而，保持应激反应基因在非应激条件下表达的机制仍然难以捉摸。最近对共阻遏物 LEUNIG_HOMOLOG (LUH) 和转录衔接蛋白 SEUSS-LIKE1 (SLK1) 和 SLK2 的遗传特征已被提议在不同的发育过程中发挥冗余作用；然而，它们在非生物胁迫反应中的功能尚不清楚。此外，LUH、SLK1 和 SLK2 的分子功能仍然不清楚。在这里，我们展示了 LUH、SLK1 和 SLK2 的分子功能以及该复合物在非生物胁迫反应中的作用。结果 luh、slk1 和 slk2 突变体植物对盐和渗透胁迫条件显示出增强的耐受性。SLK1 和 SLK2 与 LUH 中的 LUFS 结构域发生物理相互作用，形成 SLK1-LUH 和 SLK2-LUH 共阻遏物复合物以抑制转录。LUH 具有阻遏活性，而 SLK1 和 SLK2 作为接头招募 LUH，后者又将组蛋白去乙酰化酶募集到目标序列以抑制转录。应激反应基因 RD20、MYB2 和 NAC019 在 luh、slk1 和 slk2 突变植物中以升高的水平表达。此外，这些应激反应基因与 luh、slk1 和 slk2 突变植物中 H3K9 和 H3K14 的核小体密度降低和乙酰化水平增加有关。结论我们的结果表明 SLK1、SLK2 和 LUH 形成了一个共阻遏复合物。

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Involvement of co-repressor LUH and the adapter proteins SLK1 and SLK2 in the regulation of abiotic stress response genes in Arabidopsis.

BACKGROUND During abiotic stress many genes that are important for growth and adaptation to stress are expressed at elevated levels. However, the mechanisms that keep the stress responsive genes from expressing under non stress conditions remain elusive. Recent genetic characterization of the co-repressor LEUNIG_HOMOLOG (LUH) and transcriptional adaptor proteins SEUSS-LIKE1 (SLK1) and SLK2 have been proposed to function redundantly in diverse developmental processes; however their function in the abiotic stress response is unknown. Moreover, the molecular functions of LUH, SLK1 and SLK2 remain obscure. Here, we show the molecular function of LUH, SLK1 and SLK2 and the role of this complex in the abiotic stress response. RESULTS The luh, slk1 and slk2 mutant plants shows enhanced tolerance to salt and osmotic stress conditions. SLK1 and SLK2 interact physically with the LUFS domain in LUH forming SLK1-LUH and SLK2-LUH co-repressor complexes to inhibit the transcription. LUH has repressor activity, whereas SLK1 and SLK2 function as adaptors to recruit LUH, which in turn recruits histone deacetylase to the target sequences to repress transcription. The stress response genes RD20, MYB2 and NAC019 are expressed at elevated levels in the luh, slk1 and slk2 mutant plants. Furthermore, these stress response genes are associated with decreased nucleosome density and increased acetylation levels at H3K9 and H3K14 in the luh, slk1 and slk2 mutant plants. CONCLUSIONS Our results indicate that SLK1, SLK2 and LUH form a co-repressor complex. LUH represses by means of an epigenetic process involving histone modification to facilitate the condensation of chromatin thus preventing transcription at the target genes.

更新日期：2014-02-24

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