推测的液泡 Ca2+ 转运蛋白（CAXs 和 ACA）在耐盐和敏感水稻品种根中的表达和功能分析,Protoplasma

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推测的液泡 Ca2+ 转运蛋白（CAXs 和 ACA）在耐盐和敏感水稻品种根中的表达和功能分析
Protoplasma ( IF 2.5 ) Pub Date : 2014-01-31 , DOI: 10.1007/s00709-014-0615-2
Nana Yamada ₁ , Cattarin Theerawitaya , Suriyan Cha-um , Chalermpol Kirdmanee , Teruhiro Takabe

Affiliation

液泡 Ca2+-转运蛋白对水稻 (Oryza sativa L.) 根的耐盐性起重要作用。在这里，我们比较了推定的液泡阳离子/H+ 交换剂 (CAX) 和钙调素调节的自抑制 Ca2+-ATP 酶 (ACA) 在耐盐 cv. 水稻根中的表达谱。Pokkali 和盐敏感 cv。IR29。除了水稻基因组中五个假定的液泡 CAX 基因外，还注释了一个新的 CAX 基因（OsCAX4）。在本研究中，我们分离了 OsCAX4 基因，并表明其编码的蛋白质具有独特的跨膜结构，可能不仅参与运输 Ca2+，还参与运输 Mn2+ 和 Cu2+。这六个 OsCAX 基因在暴露于盐胁迫的耐受与敏感水稻品种的根中的 mRNA 表达模式不同。例如，OsCAX4 在长期盐胁迫下在 IR29（敏感）中表现出丰富的表达。还检查了四种假定的液泡 Ca2+-ATPases (OsACA4-7) 的 mRNA 表达谱。在对照条件下，OsACA4、OsACA5 和 OsACA7 的 mRNA 水平在 IR29 和 Pokkali 之间相对较高且相似。在盐胁迫下，只有 OsACA4 首先显示其在 Pokkali（耐受）中的表达降低，然后显着增加。基于这些结果，讨论了液泡 Ca2+ 转运蛋白对水稻根系耐盐性的作用。只有 OsACA4 首先显示其在 Pokkali（耐受）中的表达降低，然后显着增加。基于这些结果，讨论了液泡 Ca2+ 转运蛋白对水稻根系耐盐性的作用。只有 OsACA4 首先显示其在 Pokkali（耐受）中的表达降低，然后显着增加。基于这些结果，讨论了液泡 Ca2+ 转运蛋白对水稻根系耐盐性的作用。

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Expression and functional analysis of putative vacuolar Ca2+-transporters (CAXs and ACAs) in roots of salt tolerant and sensitive rice cultivars

Vacuolar Ca2+-transporters could play an important role for salt tolerance in rice (Oryza sativa L.) root. Here, we compared the expression profiles of putative vacuolar cation/H+ exchanger (CAX) and calmodulin-regulated autoinhibited Ca2+-ATPase (ACA) in rice roots of salt tolerant cv. Pokkali and salt sensitive cv. IR29. In addition to five putative vacuolar CAX genes in the rice genome, a new CAX gene (OsCAX4) has been annotated. In the present study, we isolated the OsCAX4 gene and showed that its encoded protein possesses a unique transmembrane structure and is potentially involved in transporting not only Ca2+ but also Mn2+ and Cu2+. These six OsCAX genes differed in their mRNA expression pattern in roots of tolerant versus sensitive rice cultivars exposed to salt stress. For example, OsCAX4 showed abundant expression in IR29 (sensitive) upon prolonged salt stress. The mRNA expression profile of four putative vacuolar Ca2+-ATPases (OsACA4-7) was also examined. Under control conditions, the mRNA levels of OsACA4, OsACA5, and OsACA7 were relatively high and similar among IR29 and Pokkali. Upon salt stress, only OsACA4 showed first a decrease in its expression in Pokkali (tolerant), followed by a significant increase. Based on these results, a role of vacuolar Ca2+ transporter for salt tolerance in rice root was discussed.

更新日期：2014-01-31

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