拟南芥中适当的体细胞重组需要多梳基组蛋白甲基转移酶CLF。,Journal of Integrative Plant Biology

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拟南芥中适当的体细胞重组需要多梳基组蛋白甲基转移酶CLF。
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2014-04-01 , DOI: 10.1111/jipb.12157
Na Chen ₁ , Wang-Bin Zhou , Ying-Xiang Wang , Ai-Wu Dong , Yu Yu

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同源重组（HR）是生殖细胞减数分裂和体细胞DNA损伤修复过程中的关键过程。尽管染色质结构被认为对HR至关重要，但到目前为止，在HR调节中仅研究了少数染色质修饰剂。在这里，我们研究了CURLY LEAF（CLF），一种负责组蛋白3赖氨酸27三甲基化（H3K27me3）的多梳基团（PcG）基因在拟南芥体细胞和减数分裂HR中的功能。尽管在花粉和种子中的荧光蛋白报告基因检测表明，功能丧失的突变体clf-29中减数分裂交换的频率与野生型没有显着差异，但HR的频率较低。在正常条件下和博来霉素治疗下，clf‐29比野生型高。DNA损伤水平之间是相当的CLF-29和野生型，即使几个DNA损伤修复基因（例如ATM，BRCA2a，RAD50，RAD51，RAD54，和PARP2）在较低的水平表达CLF-29。在博来霉素处理下，clf-29和野生型中DNA修复基因的表达水平相似，因此CLF也可以通过其他机制来调节人力资源。这些发现扩展了对PcG功能的当前了解，并有助于表观遗传调控在基因组稳定性调控中的普遍兴趣。

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Polycomb-group histone methyltransferase CLF is required for proper somatic recombination in Arabidopsis.

Homologous recombination (HR) is a key process during meiosis in reproductive cells and the DNA damage repair process in somatic cells. Although chromatin structure is thought to be crucial for HR, only a small number of chromatin modifiers have been studied in HR regulation so far. Here, we investigated the function of CURLY LEAF (CLF), a Polycomb‐group (PcG) gene responsible for histone3 lysine 27 trimethylation (H3K27me3), in somatic and meiotic HR in Arabidopsis thaliana. Although fluorescent protein reporter assays in pollen and seeds showed that the frequency of meiotic cross‐over in the loss‐of‐function mutant clf‐29 was not significantly different from that in wild type, there was a lower frequency of HR in clf‐29 than in wild type under normal conditions and under bleomycin treatment. The DNA damage levels were comparable between clf‐29 and wild type, even though several DNA damage repair genes (e.g. ATM, BRCA2a, RAD50, RAD51, RAD54, and PARP2) were expressed at lower levels in clf‐29. Under bleomycin treatment, the expression levels of DNA repair genes were similar in clf‐29 and wild type, thus CLF may also regulate HR via other mechanisms. These findings expand the current knowledge of PcG function and contribute to general interests of epigenetic regulation in genome stability regulation.

更新日期：2014-04-01

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