一对从大麦秸秆（大麦）衍生的新型抗蓝藻化感剂的手性黄酮木素：其抗蓝细菌活性的表征和比较。,Environmental Microbiology

当前位置： X-MOL 学术 › Environ. Microbiol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

一对从大麦秸秆（大麦）衍生的新型抗蓝藻化感剂的手性黄酮木素：其抗蓝细菌活性的表征和比较。
Environmental Microbiology ( IF 4.3 ) Pub Date : 2013-09-17 , DOI: 10.1111/1462-2920.12226
Xi Xiao ₁ , Haomin Huang , Zhiwei Ge , Trine B Rounge , Jiyan Shi , Xinhua Xu , Ruobing Li , Yingxu Chen

Affiliation

大麦秸秆（大麦）对蓝细菌的抑制作用已在许多领域和实验室研究中进行了30多年，尽管引起这种抗蓝细菌作用的化合物仍然未知。在这项研究中，使用针对微囊藻的生物测定指导的分离方法从大麦秸秆提取物中分离出一对手性黄酮木聚糖。通过NMR（核磁共振）和HPLC-MS（高效液相色谱-质谱）阐明化感化合物的结构，结果证明其为Salcolin A和B。对映异构体的抗蓝细菌能力不同。两种对映异构体均对微囊藻有抑制作用，Salcolin A和B的EC50（最大作用浓度的50％）分别为6.02×10（-5）和9.60×10（-5）mol l（-1），分别。此外，通过流式细胞术在单个细胞水平上研究和比较了对映体的作用方式。发现Salcolin A诱导蓝细菌细胞内ROS（活性氧）水平增加并抑制酯酶活性，而Salcolin B导致蓝细菌细胞质渗漏。因此，Salcolin A更具“止痛作用”，而Salcolin B更具“止痛作用”。这项研究表明，salcolin是大麦草对蓝藻的抑制作用中的关键化感化学物质，可以用作将来控制蓝藻有害藻华的试剂。发现Salcolin A诱导蓝细菌细胞内ROS（活性氧）水平增加并抑制酯酶活性，而Salcolin B导致蓝细菌细胞质渗漏。因此，Salcolin A更具“止痛作用”，而Salcolin B更具“止痛作用”。这项研究表明，salcolin是大麦草对蓝藻的抑制作用中的关键化感化学物质，可以用作将来控制蓝藻有害藻华的试剂。发现Salcolin A诱导蓝细菌细胞内ROS（活性氧）水平增加并抑制酯酶活性，而Salcolin B导致蓝细菌细胞质渗漏。因此，Salcolin A更具“止痛作用”，而Salcolin B更具“止痛作用”。这项研究表明，salcolin是大麦草对蓝藻的抑制作用中的关键化感化学物质，可以用作将来控制蓝藻有害藻华的试剂。

"点击查看英文标题和摘要"

A pair of chiral flavonolignans as novel anti-cyanobacterial allelochemicals derived from barley straw (Hordeum vulgare): characterization and comparison of their anti-cyanobacterial activities.

The inhibitory effect of barley straw (Hordeum vulgare) on cyanobacteria has been observed in many field and laboratory studies for over 30 years, although the compounds responsible for this anti-cyanobacterial effect have remained unknown. In this study, a pair of chiral flavonolignans were isolated from barley straw extract using a bioassay-guided isolation procedure against Microcystis sp. The structures of the allelopathic compounds were elucidated by NMR (nuclear magnetic resonance) and HPLC-MS (high performance liquid chromatography-mass spectrometry), and turned out to be salcolin A and B. The enantiomers differ in their anti-cyanobacterial abilities. Both enantiomers exhibited inhibitory effects on Microcystis sp., and the EC50 (concentration for 50% of maximal effect) of salcolin A and B were 6.02 × 10(-5) and 9.60 × 10(-5 ) mol l(-1) , respectively. Furthermore, the modes of actions of the enantiomers were investigated and compared at a single cell level by flow cytometry. Salcolin A was found to induce an increase on cyanobacterial intracellular ROS (reactive oxygen species) levels and to inhibit esterase activity, whereas salcolin B caused leakages of cyanobacterial cytoplasms. Thus, salcolin A was more 'algistatic', and salcolin B was more 'algicidal'. This study suggests that salcolin is the key allelochemical in barley straw's inhibitory effect on cyanobacteria and could be used as an agent in the future control of cyanobacterial harmful algae blooms.

更新日期：2013-08-27

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南